Technique for microinjection into eggs of the housefly, Musca domestica (Diptera: Muscidae)
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概要
- 論文の詳細を見る
A method for microinjection was developed for the housefly, Musca domestica. Chorion membranes of freshly laid eggs were first removed with sodium hypochlorite (NaOC1). Dechorionated eggs were placed on doublesided Scotch^[○!R] tape, desiccated and covered with Halocarbon oil (Sigma). Posterior ends of the eggs were pierced with a fine glass needle for microinjection. Injected eggs were incubated at 25℃ until hatching. Eggs dechorionated approximately 40 min after oviposition showed the highest hatching rate. Green food dye and suspended plasmid DNA constructs, included in the phosphate buffer, showed no harmful effects on embryonic development. Using the above procedure, an average of 75.0% of injected eggs survived to the first larval stadium. The technique described herein cna be used for microinjection of various substances into the housefly.
- 日本応用動物昆虫学会の論文
- 2000-02-25
著者
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Honda H
Univ. Tsukuba Ibaraki Jpn
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Kimura K
Laboratory Of Apiculture Department Of Animal Genetics National Institute Of Animal Industry
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HONDA Hiroshi
Laboratory of Applied Entomology, Faculty of Agriculture, University of Tokyo
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Yoshiyama Mikio
Laboratory of Applied Zoology, Institute of Agriculture and Forestry, University of Tsukuba
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Kimura Kiyoshi
Laboratory of Apiculture, Department of Animal Genetics, National Institute of Animal Industry
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Kimura Kiyoshi
Laboratory Of Apiculture Department Of Animal Genetics National Institute Of Animal Industry
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Honda Hiroshi
Laboratory Of Applied Entomology Faculty Of Agriculture University Of Tokyo
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Yoshiyama Mikio
Laboratory Of Applied Zoology Institute Of Agriculture And Forestry University Of Tsukuba
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Honda Hiroshi
Laboratory Of Applied Entomology Faculty Of Agriculture The University Of Tokyo
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