Hepatocyte Spheroids Underlayered with Nonparenchymal Cells for Biomedical Applications
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概要
- 論文の詳細を見る
A two-dimensional microarray of ten thousand (100×100) hepatocyte hetero-spheroids, underlaid with non-parenchymal cells, was successfully constructed with a 100-µm spacing on micro-fabricated glass substrates that were coated with poly(ethylene glycol) (PEG). Co-cultivation of hepatocytes with endothelial cells was essential to stabilize hepatocyte viability and liver-specific functions, allowing us to obtain hepatocyte spheroids with a diameter of 100-µm, functioning as a miniaturized liver to secrete albumin for at least 1 month. The most important feature of this study is that these substrates are defined to provide an unprecedented control of substrate properties for modulating cell behavior, employing both surface engineering and synthetic polymer chemistry. The spheroid array constructed here is highly useful as a platform of tissue and cell-based biosensors (TBB and CBB), detecting a wide variety of clinically, pharmacologically, and toxicologically active compounds through a cellular physiological response.
- （社）電子情報通信学会の論文
- 2011-02-01
著者
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NAKASONE Yuichi
Department of Applied Chemistry, Tokyo University of Science
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TATEISHI Tetsuya
Department of Applied Chemistry, Tokyo University of Science
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OTSUKA Hidenori
Department of Applied Chemistry, Tokyo University of Science
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TATEISHI Tetsuya
National Institute for Advanced Interdisciplinary Research
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Otsuka Hidenori
Department Of Applied Chemistry And Institute Of Colloid And Interface Science Science University Of
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Tateishi Tetsuya
Biomaterials Center National Institute For Material Science
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Tateishi Tetsuya
Crest Jst:univ. Tokyo
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Tateishi Tetsuya
Tissue Engineering Research Center National Institute Of Advanced Industrial Science And Technology:
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Tateishi Tetsuya
Mechanical Engineering Laboratory Agency Of Industrial Science And Technology Ministry Of Internatio
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Tateishi T
National Inst. Advanced Interdisciplinary Res. Ibaraki Jpn
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Tateishi T
Department Of Mechanical Engineering Graduate School Of Engineering The University Of Tokyo
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Yamamoto Masashi
Department Of Chemical Science And Engineering Faculty Of Engineering Kobe University
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Tateishi Tetsuya
Department Of Applied Chemistry Tokyo University Of Science
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Tateishi Tetsuya
Biomaterials Center National Institute For Materials Science
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Tateishi Tetsuya
Biomedical Engineering Laboratory (tateishi & Ushida Laboratory) Department Of Mechanical Engine
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Tateishi Tetsuya
Graduate School Of Engineering University Of Tokyo
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Tateishi Tetsuya
Mechanical Engineering Laboratory Agency Of Industrial Science And Technology Ministry Of Internatio
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Otsuka Hidenori
Department Of Applied Chemistry Tokyo University Of Science
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Nakasone Yuichi
Department Of Applied Chemistry Tokyo University Of Science
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Yamamoto Masashi
Department Of Applied Chemistry Tokyo University Of Science
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