Modulation of Substrate Preference of Thermus Maltogenic Amylase by Mutation of the Residues at the Interface of a Dimer
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概要
- 論文の詳細を見る
To elucidate the relationship between the substrate size and geometric shape of the catalytic site of Thermus maltogenic amylase, Gly50, Asp109, and Val431, located at the interface of the dimer, were replaced with bulky amino acids. The kcat⁄Km value of the mutant for amylose increased significantly, whereas that for amylopectin decreased as compared to that of the wild-type enzyme. Thus, the substituted bulky amino acid residues modified the shape of the catalytic site, such that the ability of the enzyme to distinguish between small and large molecules like amylose and amylopectin was enhanced.
- 社団法人 日本農芸化学会の論文
- 2007-06-23
著者
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LEE Byong
Department of Biomedical Engineering and Materials, School of Medicine, Soonchunhyang University
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Park K‐h
Chosun Univ. Kwangju Kor
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PARK Sung-Hoon
Department of Laboratory Animal Medicine, College of Veterinary Medicine and KRF Zoonotic Disease Pr
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Park Keun-hyung
Department Of Food Science & Technology And Institute Of Agricultural Science & Technology
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PARK Kwan-Hwa
Center for Agricultural Biomaterials, National Laboratory for Functional Food Carbohydrates, and Dep
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Park K‐h
Seoul National Univ. Suwon Kor
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Park Kwan-hwa
Center For Agricultural Biomaterials National Laboratory For Functional Food Carbohydrates And Depar
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Park Kwan-hwa
Department Of Food Science And Technology College Of Agriculture And Life Sciences Seoulnational Uni
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Park Kwan-hwa
Center For Agricultural Bio-materials And Department Of Food Science And Technology School Of Agricu
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Park Kwan-hwa
Center For Agricultural Biomaterials And Department Of Food Science And Biotechnology School Of Agri
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Oh Byung-ha
Department Of Macromolecular Sciences Smithkline Beecham Pharmaceuticals King Of Prussia
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Park Sung-hoon
Department Of Chemical Engineering Pusan National University
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KANG Hee-Kwon
Center for Agricultural Biomaterials and Department of Food Science and Biotechnology, School of Agr
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CHA Hyunju
Center for Agricultural Biomaterials and Department of Food Science and Biotechnology, School of Agr
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KIM Jung-Wan
Department of Biology, University of Incheon
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SHIM Jae-Hoon
Center for Agricultural Biomaterials and Department of Food Science and Biotechnology, College of Ag
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WOO Eui-Jeon
Systemic Proteomics Research Center, Korea Research Institute of Bioscience and Biotechnology
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HONG Jung-Sun
Systemic Proteomics Research Center, Korea Research Institute of Bioscience and Biotechnology
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Kim Jung-wan
The Research Center For New Biomaterials In Agriculture And Department Of Food Science And Technolog
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Cha Hyunju
Center For Agricultural Biomaterials And Department Of Food Science And Biotechnology School Of Agri
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Kim Jung-wan
Department Of Biology University Of Incheon
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Woo Eui-jeon
Systemic Proteomics Research Center Korea Research Institute Of Bioscience And Biotechnology
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Hong Jung-sun
Systemic Proteomics Research Center Korea Research Institute Of Bioscience And Biotechnology
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Kang Hee-kwon
Center For Agricultural Biomaterials And Department Of Food Science And Biotechnology School Of Agri
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Shim Jae-hoon
Center For Agricultural Biomaterials And Department Of Food Science And Biotechnology College Of Agr
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Park Sung-hoon
Department Of Food Science And Agricultural Chemistry Mcgill University
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Lee Byong
Department Of Biomedical Engineering And Materials Soonchunhyang University
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Oh Byung-Ha
Department of Life Science, School of Environmental Engineering, Pohang University of Science and Technology
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