Detection of Early Pregnancy Factor (EPF) in Mare Sera
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概要
- 論文の詳細を見る
Early Pregnancy Factor (EPF) has been detected in the pregnant sera of almost all the species examined. Yet no EPF of mare sera had been reported prior to this study, because the method of examination had not yet been established. The present study was carried out to examine various factors which influence the rosette inhibition test of mares and to establish a reliable assay system for Rosette Inhibition Titre (RIT). The following items were found optimal for the detection of RIT in mare sera: human erythrocytes (red blood cells) showed the best reactivity among human, sheep, and rat erythrocytes; the ratio of lymphocytes to erythrocytes of 1:20 was proven to show better results; and Guinea Pig Complement (GPC) was not necessary. When the rosette inhibition test was performed on 10 pregnant and 9 nonpregnant sera, the RIT values ranged from 6 to 8 (7.2 ± 1.0) in nonpregnant sera, and from 9 to 12 (9.5 ± 1.0) in pregnant sera, with a significant difference (p<0.01). These results suggested the availability of the RIT for pregnant mare sera as an index of early detection of pregnancy in mare.
- 日本繁殖生物学会の論文
- 1996-02-01
著者
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Miyake Y
Laboratory Of Theriogenology Faculty Of Agriculture Iwate University
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Miyake Yoh-ichi
Laboratory Of Theriogenology Department Of Veterinary Medicine Faculty Of Agriculture Iwate Universi
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Takahashi J
Laboratory Of Animal Breeding And Reproduction Faculty Of Agriculture Iwate University
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TAKAHASHI Jutaro
Laboratory of Animal Breeding, Faculty of Agriculture, Iwate University, Morioka 020, Japan
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YASUDA Yasuhisa
Laboratory of Animal Breeding, Faculty of Agriculture, Iwate University, Morioka 020, Japan
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Ohnuma Katsuhiko
Laboratory Of Animal Breeding Faculty Of Agriculture Iwate University
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ITO Kazuei
Laboratory of Animal Reproduction, Akita Prefectural College of Agriculture
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Ito K
Laboratory Of Animal Breeding And Reproduction Faculty Of Agriculture Iwate University
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Yasuda Y
Laboratory Of Animal Breeding And Reproduction Faculty Of Agriculture Iwate University
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Yasuda Yasuhisa
Laboratory Of Animal Breeding Iwate University
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TAKAHASHI Jutaro
Laboratory of Animal Breeding and Reproduction, Faculty of Agriculture, Iwate University
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YASUDA Yasuhisa
Laboratory of Animal Breeding and Reproduction, Faculty of Agriculture, Iwate University
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Ito Kazuei
Laboratory of Animal Breeding and Reproduction, Faculty of Agriculture, Iwate University
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