Isolation and Purification of Early Pregnancy Factor (EPF) in Rat Serum
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概要
- 論文の詳細を見る
Early pregnancy factor (EPF) has been detected in pregnancy sera of many animal species. Isolation and purification of the EPF in pregnancy rat serum (Day 4) was attempted by using ammonium sulfate fractionation, ion-exchange chromatography, affinity chromatography and Immunoblotting. As a result, rat EPF was detected in 40% ammonium sulfate supernatant, CM-Sepharose Fast Flow 20 mM and 30 mM NaCl elution fraction, DEAE-Sepharose Fast Flow unbound fraction and Affinity chromatography unbound fraction. By the result of immunoblotting, there were three bands (22 KD, 24 KD and 28 KD) in the purified rat EPF fraction.
- 日本繁殖生物学会の論文
- 1996-02-01
著者
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TAKAHASHI Jutaro
Laboratory of Animal Breeding, Faculty of Agriculture, Iwate University, Morioka 020, Japan
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Ikemizu Y
Laboratory Of Animal Breeding Faculty Of Agriculture Iwate University
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Takahashi J
Laboratory Of Animal Breeding Iwate University
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Ikemizu Yoshimi
Laboratory Of Animal Breeding Faculty Of Agriculture Iwate University
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Yamazaki Satoru
Laboratory Of Animal Breeding Faculty Of Agriculture Iwate University
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Yasuda Y
Laboratory Of Animal Breeding And Reproduction Faculty Of Agriculture Iwate University
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Yasuda Yasuhisa
Laboratory Of Animal Breeding Iwate University
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TAKAHASHI Jutaro
Laboratory of Animal Breeding and Reproduction, Faculty of Agriculture, Iwate University
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YASUDA Yasuhisa
Laboratory of Animal Breeding and Reproduction, Faculty of Agriculture, Iwate University
関連論文
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- Quantitative Analysis of Coat-color Patterns in BALB/c ⇔ C57BL/6 Mouse Chimeras by Means of a Computer-Based Color Image Processor System
- Expression of the Myogenin-LacZ Reporter Gene During Early Postimplantation Development of the Mouse
- Function of the Myogenin Gene Promoter in the Muscular Dystrophic Transgenic dy/dy Mouse
- Detection of Early Pregnancy Factor (EPF) in Mare Sera
- 胚幹細胞に導入されたPMGN(-4K)LacZ-neo遺伝子のin vitroにおける発現
- Isolation and Purification of Early Pregnancy Factor (EPF) in Rat Serum
- Release Period of EPF-Like Substance(s) in Rat and Bovine Fertilized Ova
- Influence of Anti-Bovine Early Pregnancy Factor Antibody on Embryonic Development and Implantation in Rats
- EPF as a Marker for Early Embryonic Losses in Repeat Breeders
- Early Pregnancy Factor : EPF-like Substance(s) Purified from Pregnant Bovine Ovary and In Vitro Fertilized Ovum Culture Medium
- Early Pregnancy Factor: Its Characterization and Purification from Pregnant Swine Serum
- Bovine Early Pregnancy Factor: Its Characterization and an Attempt to Produce Anti-Bovine EPF Antibody
- Study of EPF-Like Substance(s) Detected in Fertilized Bovine Ovum Culture Medium