Early Pregnancy Factor : EPF-like Substance(s) Purified from Pregnant Bovine Ovary and In Vitro Fertilized Ovum Culture Medium

スポンサーリンク

概要

• 論文の詳細を見る

• The objective of the present investigation was to purify early pregnancy factor (EPF)-like substance(s) from a pregnant bovine ovarian extract and in vitro fertilized ovum culture medium (IVF-OCM). Both the ovarian extract and IVF-OCM had EPF-like activity. These EPF-like substance(s) bound with CM-Sepharose and CNBr-activated Sepharose coupled with anti-EPF IgG but did not bind with a HPLC-DEAE column. The EPF-like substance(s) purified from the ovary and IVF-OCM had EPF-like activity (RIT≥5) at M.W. 17-38 KD and M.W. 21-30 KD, respectively. SDS-PAGE analysis revealed that the ovarian extract and IVF-OCM positive fractions had a band of 21.5 KD similar to that of EPF purified from pregnant bovine serum. Antiserum to HPLC fraction III (IVF-OCM origin, RIT≥5) decreased the RIT value of pregnant bovine serum from 6 to 3 and reacted with a band of 21.5 KD protein purified from bovine pregnant serum. Therefore, these two EPF-like substance(s) and EPF from pregnant serum may be similar substances.

• 日本繁殖生物学会の論文
• 1995-02-01

著者

• Kawahata K

  Koiwai Noboku Co. Ltd. Iwate Jpn

• Goto T

  Koiwai Noboku Co. Ltd. Iwate Jpn

• Takahashi J

  Laboratory Of Animal Breeding And Reproduction Faculty Of Agriculture Iwate University

• TAKAHASHI Jutaro

  Laboratory of Animal Breeding, Faculty of Agriculture, Iwate University, Morioka 020, Japan

• Ikemizu Y

  Laboratory Of Animal Breeding Faculty Of Agriculture Iwate University

• Goto Taichi

  Technical Research Center Koiwai Noboku Co. Ltd.

• Kawahata Kyoko

  Technical Research Center Koiwai Noboku Co. Ltd.

• Ikemizu Yoshimi

  Laboratory Of Animal Breeding Faculty Of Agriculture Iwate University

• ITO Kazuei

  Laboratory of Animal Reproduction, Akita Prefectural College of Agriculture

• Ito K

  Laboratory Of Animal Breeding And Reproduction Faculty Of Agriculture Iwate University

• Yasuda Y

  Laboratory Of Animal Breeding And Reproduction Faculty Of Agriculture Iwate University

• Yasuda Yasuhisa

  Laboratory Of Animal Breeding Iwate University

• TAKAHASHI Jutaro

  Laboratory of Animal Breeding and Reproduction, Faculty of Agriculture, Iwate University

• YASUDA Yasuhisa

  Laboratory of Animal Breeding and Reproduction, Faculty of Agriculture, Iwate University

• Ito Kazuei

  Laboratory of Animal Breeding and Reproduction, Faculty of Agriculture, Iwate University

関連論文

• Detection of Early Pregnancy Factor in the Sera of Cows Whose Uteri were Infused with Trophoblastic Cell Culture Medium
• ホルスタイン種産子における中手幅および中足幅と生時体重の関係
• Function of the Myogenin Gene Promoter in the Muscular Dystrophic Transgenic dy/dy Mouse
• Expression of the Myogenin-LacZ Reporter Gene During Early Postimplantation Development of the Mouse
• Quantitative Analysis of Coat-color Patterns in BALB/c ⇔ C57BL/6 Mouse Chimeras by Means of a Computer-Based Color Image Processor System
• Quantitative Analysis of Coat-color Patterns in BALB/c ⇔ C57BL/6 Mouse Chimeras by Means of a Computer-Based Color Image Processor System
• Expression of the Myogenin-LacZ Reporter Gene During Early Postimplantation Development of the Mouse
• Detection of Bovine Early Pregnancy Factor in the Culture Medium of Sperm-Injected or Plasmamembrane-Perforated Oocytes
• Bovine Early Pregnancy Factor in Recipient Serum with Embryo Transfer on the Day More Than 5 Days Different from the Estrus Cycle of Donor
• Function of the Myogenin Gene Promoter in the Muscular Dystrophic Transgenic dy/dy Mouse
• Detection of Early Pregnancy Factor (EPF) in Mare Sera
• 胚幹細胞に導入されたPMGN(-4K)LacZ-neo遺伝子のin vitroにおける発現
• Isolation and Purification of Early Pregnancy Factor (EPF) in Rat Serum
• Release Period of EPF-Like Substance(s) in Rat and Bovine Fertilized Ova
• Influence of Anti-Bovine Early Pregnancy Factor Antibody on Embryonic Development and Implantation in Rats
• EPF as a Marker for Early Embryonic Losses in Repeat Breeders
• Early Pregnancy Factor : EPF-like Substance(s) Purified from Pregnant Bovine Ovary and In Vitro Fertilized Ovum Culture Medium
• Early Pregnancy Factor: Its Characterization and Purification from Pregnant Swine Serum
• Detection of Early Pregnancy Factor in the Sera of Cows Whose Uteri were Infused with Trophoblastic Cell Culture Medium
• Bovine Early Pregnancy Factor: Its Characterization and an Attempt to Produce Anti-Bovine EPF Antibody
• Study of EPF-Like Substance(s) Detected in Fertilized Bovine Ovum Culture Medium

もっと見る 閉じる

スポンサーリンク