Detection of Early Pregnancy Factor in the Sera of Cows Whose Uteri were Infused with Trophoblastic Cell Culture Medium
スポンサーリンク
概要
- 論文の詳細を見る
The aim of this study was to examine whether or not uterine infusion with a medium showing positive early pregnancy factor (EPF) activity influences maternal physiology in cattle. Trophoblastic cells derived from 12-day-old bovine embryos were cultured in Dulbeccos PBS (+) at 39 C in air for 24 h. EPF activity was assayed using the rosette inhibition test. A rosette inhibition titer (RIT) above 4 was regarded as indicating positive EPF activity. The trophoblastic cell culture medium (Tro-CM) showed positive EPF activity. On days 7 to 12 after estrus, five cows each received a nonsurgical infusion of Tro-CM (0.25 ml) in the lumen of the uterine horn. Blood samples were collected daily by the tail artery or vein puncture from day 0 (the day of infusion) to day 5. All five cows showed positive EPF activity in their sera on days 2 to 4, and three cows out of the five kept showing positive EPF activity for 2 to 3 days. The result demonstrated that sera of cows infused with a small amount of EPF-positive medium (Tro-CM) into the uterus showed temporary positive EPF activity in maternal serum, further suggesting that EPF released from Tro-CM would induce a release of maternal EPF, but not by directly entering into the maternal blood flow.
- 日本繁殖生物学会の論文
- 1998-02-01
著者
-
Goto Taichi
Technical Research Center Koiwai Noboku Co. Ltd.
-
Kawahata Kyoko
Technical Research Center Koiwai Noboku Co. Ltd.
関連論文
- ホルスタイン種産子における中手幅および中足幅と生時体重の関係
- Detection of Bovine Early Pregnancy Factor in the Culture Medium of Sperm-Injected or Plasmamembrane-Perforated Oocytes
- Bovine Early Pregnancy Factor in Recipient Serum with Embryo Transfer on the Day More Than 5 Days Different from the Estrus Cycle of Donor
- Release Period of EPF-Like Substance(s) in Rat and Bovine Fertilized Ova
- EPF as a Marker for Early Embryonic Losses in Repeat Breeders
- Early Pregnancy Factor : EPF-like Substance(s) Purified from Pregnant Bovine Ovary and In Vitro Fertilized Ovum Culture Medium
- Detection of Early Pregnancy Factor in the Sera of Cows Whose Uteri were Infused with Trophoblastic Cell Culture Medium
- Study of EPF-Like Substance(s) Detected in Fertilized Bovine Ovum Culture Medium