Identification of Rat Liver Aldehyde Oxidase across Species by Accurate Peptide-Mass Fingerprinting and Sequence-Tagging with Fourier Transform Ion Cyclotron Resonance Mass Spectrometry (プロテオーム)
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概要
- 論文の詳細を見る
We evaluated applicability of Fourier transform ion cyclotron resonance mass spectrometry (FT-ICR MS) to protein identification across species. Nano-electrospray FT-ICR MS enabled acquisition of accurate mass spectra of protein digests at a picomole level sensitivity. Peptide mass maps with a ppm level accuracy were routinely obtained by spraying whole protein digests without internal mass calibrant. The accurate peptide mass maps allowed narrowing of database search-windows for peptide-mass fingerprinting and the search noise was substantially reduced. This method of protein identification was applied to a rat liver protein, which had not been sequenced previously but had been biochemically characterized as an aldehyde oxidase. The rat liver protein was successfully correlated to human and bovine counterparts across the species boundaries by peptide-mass fingerprinting with an accurate tryptic mass map. Identity of the rat protein was further confirmed by accurate fragment-ion masses obtained by hexapole-storage-assisted capillary-skimmer dissociation of a peptide mixture.
- 日本質量分析学会の論文
- 2000-06-01
著者
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中村 健道
理化学研究所物質構造解析チーム
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中村 健道
理研・バイオ解析
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中村 健道
三共・分析代謝研
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中村 健道
理化学研究所先端技術開発支援センターバイオ解析チーム
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中村 健道
理研
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中村 健道
分析代謝研究所
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KOSAKA Toshiyuki
Biomedical Research Laboratories, Sankyo Co., Ltd.
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Watanabe Nobuaki
Drug Metabolism And Pharmacokinetics Research Laboratories Daiichi-sankyo Co. Ltd.
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中村 健道
理化学研究所先端技術開発支援センター
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Kosaka T
Biomedical Research Laboratories Sankyo Co. Ltd.
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Kosaka Toshiyuki
Biomedical Research Laboratories Sankyo Co. Ltd.
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TAKAZAWA Tomoko
Biomedical Research Laboratories, Sankyo Co., Ltd.
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KUBOTA Kazuishi
Biomedical Research Laboratories, Sankyo Co., Ltd.
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NAKAMURA Takemichi
Biomedical Research Laboratories, Sankyo Co., Ltd.
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Kubota K
Biomedical Research Laboratories Sankyo Co. Ltd.
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Kubota Kazuishi
Biomedical Research Laboratories Sankyo Co. Ltd.
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Watanabe Nobuaki
Drug Metabolism And Pharmacokinetics Research Laboratories Sankyo Co. Ltd.
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Takazawa Tomoko
Biomedical Research Laboratories Sankyo Co. Ltd.
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