二次元電気泳動と質量分析法を用いたラット肝臓蛋白質の解析

概要

論文の詳細を見る
Proteome analysis exploits advanced two-dimensional gel electrophoresis (2-DE) technology and efficient methods for protein identification supported by modern mass spectrometry, and now becomes a very common tool for functional genomics, drug-target discovery, etc. In this paper, a procedure of proteome analysis is described with an example of the analysis of varied protein expression related to a disease. The protein expression patterns in Zucker diabetic fatty (ZDF) rat, which is a model of non-insulin-dependent diabetes mellitus, and lean ZDF control rat livers were displayed with 2-DE and the differences were analyzed. Two proteins which showed significant change in the protein expression, were identified by a combination of in-gel digestion, matrix-assisted laser desorption/ionization time-of-flight mass spectrometry and database search with peptide-mass fingerprinting.
日本質量分析学会の論文
1999-06-01