P-15 化学遺伝学研究用ツールとしての低分子化合物の光クロスリンク反応 : 低分子マイクロアレイと低分子アフィニティービーズへの適用(ポスター発表の部)

概要

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Recently, we reported a coupling method that enables the introduction of a variety of small molecules onto glass slides by using a photoaffinity reaction. Using this method, aryl diazirine groups covalently-introduced on glass slides are promoted upon UV irradiation to highly reactive carbene, which in turn binds or inserts irreversibly to a proximal small molecule in a functional group-independent manner. This method, referred to as photo-cross-linking, is thought to be useful in the construction of microarrays of small molecules. It is expected that a similar chemical approach can be used to introduce small molecules onto affinity gel, such as onto agarose beads. Herein we reported our recent efforts to evaluate the product diversity of the photo-cross-linking process. Construction of the photo-cross-linked small molecule microarrays by using a 2011 chemical library, and preparation of the photo-cross-linked small molecule affinity beads are also demonstrated.
天然有機化合物討論会の論文
2005-09-15