天然物構造決定におけるタンデム質量分析法の役割
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概要
- 論文の詳細を見る
Unlike NMR, mass spectrometry seems to be used as a supplementary method in general, rather than a critical tool in the structural analysis of “truly unknown” compounds, such as newly isolated biologically active natural products. One of the reasons is that instruments designed for structural analysis tend to be relatively expensive and hard to maintain and/or operate by natural product chemists, until recently. However, another, and more essential reason is underlying the nature of mass spectrometric structural analysis, i.e., the process of analysis relies on non-quantitative, highly empirical, and hard-to-generalize fragmentation rules, and is difficult to explain deductively to non-experts. It may also be believed to be difficult to extract structural information from high-energy collision induced dissociation (CID) mass spectra generated by sector-based tandem mass spectrometers, which are basically rich in structural information but hard to interpret. In this situation, we have learned that, at least in some cases, tandem mass spectrometry (MS/MS) can be used as an aid to extract vital structural information unambiguously and can be used for building unknown structures rationally, without using inspiration of an expert. In this paper, examples from our experiences in structural elucidation of natural products are shown and following points, (1) how MS/MS can help extracting crucial structural information from complex structures and (2) how MS/MS can be used for rational building of a new structure, are focused. (3) A possible role of high-energy-CID MS3 in structural elucidation of natural products is also discussed.
- 日本質量分析学会の論文
- 1997-06-01
著者
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中村 健道
理化学研究所物質構造解析チーム
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中村 健道
理研・バイオ解析
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中村 健道
三共・分析代謝研
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中村 健道
理化学研究所先端技術開発支援センターバイオ解析チーム
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中村 健道
理研
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中村 健道
分析代謝研究所
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中村 健道
三共株式会社分析代謝研究所
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中村 健道
理化学研究所先端技術開発支援センター
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