Electron Capture Dissociation of Triantennary Complex-Type N-Glycosylated Peptides: A Case of Suppressed Peptide Backbone Cleavage
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概要
- 論文の詳細を見る
We investigated the electron capture dissociation (ECD) of triantennary complex-type N-glycosylated peptides prepared from bovine fetuin. Previous reports on Fourier-transform ion cyclotron resonance mass spectrometry (FT-ICR-MS) suggested that ECD is an advantageous method for structural characterization of glycopeptides, because it can cleave the N-Cα bond on the peptide backbone while retaining the labile glycosidic bonds. We present here new data from ECD which resulted in the degradation of the glycan structure prior to any backbone cleavage. Based on the ECD experiment on deglycosylated samples, the character of the peptide backbone sequence, which contains carbamoylmethylated cysteine residue, seemed to prevent extensive N-Cα bond cleavage. It appears that more basic investigations are necessary to promote practical use of ECD for structural characterization of complex glycopeptides because each type of glycopeptide exhibits particular fragmentation patterns.
- 日本質量分析学会の論文
著者
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中村 健道
理化学研究所物質構造解析チーム
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中村 健道
理研・バイオ解析
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中村 健道
三共・分析代謝研
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中村 健道
理化学研究所先端技術開発支援センターバイオ解析チーム
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Nakamura Takemichi
Biomolecular Characterization Team, RIKEN
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中村 健道
理化学研究所先端技術開発支援センター
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SATO Akihiro
JASCO International Corporation Limited
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HONGO Yayoi
Molecular Characterization Team, RIKEN
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Hongo Yayoi
Molecular Characterization Team Riken
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Sato Akihiro
Jasco International Co. Ltd.
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