トリメチルシリル誘導体の高速原子衝撃質量分析法及びタンデム質量分析法への応用(<特集>質量分析法の応用技術)

概要

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The effect of trimethylsilyl (TMS) derivatives, which have been long and widely used especially in gas chromatography/mass spectrometry (GC/MS), was examined with fast atom bombardment (FAB) mass spectrometry. In order to investigate what kind of information can be obtained from FABMS of TMS derivatives of biologically related compounds, FABMS and FAB MS/MS (tandem mass spectrometry) of TMS derivatives of compounds such as 2'-O-methyladenosine, 3'-O-methyladenosine, guanosine, uridine and some amino acids were studied. As a result, FABMS spectra of trimethylsilylated compounds gave molecular ion peaks showing that the TMS groups attached to alcoholic hydroxy groups remained intact, while the TMS groups attached to NH, SH, COOH and amide groups were labile and partly removed. It was concluded that FABMS spectra of trimethylsilylated substances have the advantage of not only determining the total number of active hydrogens, but also differentiating groups such as OH, OH, SH, CONH and COOH. It was also proved that FAB mass spectrometry of TMS derivatives of biologically related compounds are very useful for structural analysis of the native compounds. Here we have mainly used 3-nitrobenzyl alcohol (3-NBA) as the matrix for FABMS because of its solubility in the trimethylsilylation reaction mixture.
社団法人日本分析化学会の論文
1996-06-05