Pharmacokinetic Study of β-Methyldigoxin by Enzyme Immunoassay Using a Novel Specific Antiserum in Rats
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概要
- 論文の詳細を見る
We previously showed that enzyme immunoassay (EIA) of β-methyldigoxin (MDx3) using anti-MDx3 3'-hemisuccinate-bovine serum albumin antiserum (Antiserum-I) was superior to that using commercial antidigoxin antiserum (Antiserum-II) in terms of specificity and that pretreatment of human serum with phenyl boric acid (PBA) column was effective. In the present study, we examined the precision of EIA using Antiserum-I and the recovery of MDx3 after PBA column treatment in rat serum, and also investigated pharmacokinetic changes of MDx3 in rats. The intra-and inter-assay variations and recovery tests using Antiserum-I were good. The PBA column was effective in selectively separating MDx3 from rat serum containing MDx3 and its metabolites. The recovery tests using Antiserum-I with PBA column showed about 110% and the interference of metabolites of MDx3 was negligible. Serum concentration-time courses of MDx3 by EIA using Antiserum-I with PBA column and Antiserum-I were lower than that using Antiserum-II. The distribution volume at steady state and total body clearance values of MDx3 in these conditions were significantly higher than those using Antiserum-II. The usefulness of PBA column was ascertained, while effects of PBA column on these parameters were not significant. In addition, rapid absorption of MDx3 was observed by EIA using Antiserum-I with PBA column. These results suggest that EIA using Antiserum-I with PBA column for the pretreatment of serum samples should be a more useful and valuable system in therapeutic drug monitoring and pharmacokinetic studies of the unchanged type of MDx3 than Antiserum-II.
- 公益社団法人日本薬学会の論文
- 2003-02-01
著者
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HIGASHI Yasuhiko
Department of Analytical Chemistry, Faculty of Pharmaceutical Sciences, Hokuriku University
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FUJII Youichi
Department of Analytical Chemistry, Faculty of Pharmaceutical Sciences, Hokuriku University
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Fujii Y
Department Of Analytical Chemistry Faculty Of Pharmaceutical Sciences Hokuriku University
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Fujii Youichi
Department Of Analytical Chemistry Faculty Of Pharmaceutical Sciences Hokuriku University
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Watanabe Nobuaki
Drug Metabolism And Pharmacokinetics Research Laboratories Daiichi-sankyo Co. Ltd.
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Watanabe N
Pharmacokinetics And Drug Delivery Research Laboratories Sankyo Co. Ltd.
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Watanabe Naomi
Department Of Analytical Chemistry Faculty Of Pharmaceutical Sciences Hokuriku University
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Higashi Yasuhiko
Department Of Analytical Chemistry Faculty Of Pharmaceutical Sciences Hokuriku University
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Higashi Y
Department Of Analytical Chemistry Faculty Of Pharmaceutical Sciences Hokuriku University
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SASAKI Toshiyuki
Department of Analytical Chemistry, Faculty of Pharmaceutical Sciences, Hokuriku University
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Fujii Youichi
Fac. Of Pharmaceutical Sciences Hokuriku Univ.
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Sasaki Toshiyuki
Department Of Analytical Chemistry Faculty Of Pharmaceutical Sciences Hokuriku University
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Watanabe Nobuaki
Drug Metabolism and Pharmacokinetics Research Laboratories, Daiichi-Sankyo Co., Ltd.
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