Sensitive Determination of 4-(4-Bromophenyl)-4-hydroxypiperidine, a Metabolite of Bromperidol, in Rat Plasma by HPLC with Fluorescence Detection after Pre-column Derivatization Using 4-Fluoro-7-nitro-2,1,3-benzoxadiazole(Analytical Biochemistry)
スポンサーリンク
概要
- 論文の詳細を見る
The purpose of this study was to determine the level of 4-(4-bromophenyl)-4-hydroxypiperidine (BPHP), a bromperidol (BRO) metabolite, in rat plasma by HPLC with fluorescence detection after pre-column derivatization using 4-fluoro-7-nitro-2,1,3-benzoxadiazole (NBD-F). After basic extraction of the samples with benzene, derivatization with NBD-F was conducted in borate buffer (pH 8.0) at 60℃ for 3 min. Mexiletine was utilized through the procedure as an internal standard (IS). Retention times of the BPHP and IS derivatives were 7.7 and 11.5 min, respectively. The regression equation for BPHP showed good linearity in the range of 0.01-1mg/ml with the detection limit of 0.003μg/ml. The coefficient of variation was less than 12.0%. The recovery was satisfactory. This method was applied for a pharmacokinetic study of BPHP in comparison with 4-(4-chlorophenyl)-4-hydroxypiperidine (CPHP), the corresponding haloperidol (HAL) metabolite, in rats. The ratio of the area under the plasma concentration curve (AUC) after p.o. administration of BPHP to the AUC after i.p. administration of BPHP (46%) was lower than that of CPHP (56%), indicating that intestinal absorption of BPHP is lower than that of CPHP. The ratio of BRO metabolism to BPHP (48%) was 1.8-fold higher than that of HAL metabolism to CPHP (27%); the ratio was estimated as (AUC_<p.o.,A→B>/AUC_<p.o.,B>)×100, where AUC_<p.o.,A→B> is the AUC value of BPHP or CPHP after p.o. administration of BRO or HAL, and AUC_<p.o.,B> is the AUC of BPHP or CPHP after administration of BPHP or CPHP, respectively. Our method provides a sensitive procedure for determination of BPHP in rat plasma and is suitable for pharmacokinetic studies of BPHP after BRO administration.
- 2006-12-01
著者
-
Nakamura Shota
Department of Cardiology, Fukuoka Tokusyukai Medical Center
-
HIGASHI Yasuhiko
Department of Analytical Chemistry, Faculty of Pharmaceutical Sciences, Hokuriku University
-
FUJII Youichi
Department of Analytical Chemistry, Faculty of Pharmaceutical Sciences, Hokuriku University
-
Fujii Youichi
Department Of Analytical Chemistry Faculty Of Pharmaceutical Sciences Hokuriku University
-
Nakamura Shota
Department Of Analytical Chemistry Faculty Of Pharmaceutical Sciences Hokuriku University
-
Higashi Yasuhiko
Department Of Analytical Chemistry Faculty Of Pharmaceutical Sciences Hokuriku University
関連論文
- FRS-004 The Effect of Edaravone on Plasma Monocyte Chemoattractant Protein-1 Levels in Patients with Acute Myocardial Infarction(Acute Coronary Syndrome, The 71st Annual Scientific Meeting of the Japanese Circulation Society)
- PE-307 Diabetic Mellitus was an Independent Predictor Involved in Restenosis Sirolimus-eluting Stent Implantation(Restenosis, basic/clinical-4 (IHD) PE52,Poster Session (English),The 70th Anniversary Annual Scientific Meeting of the Japanese Circulation S
- PE-093 DRIVER, Cobalt Alloy Stent, Can be a Positive Choice in Non-DM Patients with Low TLR Risk Even in DES Era(Coronary revascularization, PCI-10 (IHD) PE16,Poster Session (English),The 70th Anniversary Annual Scientific Meeting of the Japanese Circulat
- Sensitive Determination of 4-(4-Bromophenyl)-4-hydroxypiperidine, a Metabolite of Bromperidol, in Rat Plasma by HPLC with Fluorescence Detection after Pre-column Derivatization Using 4-Fluoro-7-nitro-2,1,3-benzoxadiazole(Analytical Biochemistry)
- Possible involvement of Atypical Protein Kinase C in Aldosterone-induced-Angiotensin-Converting-Enzyme Gene Expression in Cultured Neonatal Rat Cardiocytes
- OJ-327 Fluvastatin Increases Transcriptional Activity of the Endothelial Nitric Oxide Synthase Gene, Especially With -786T/C Polymorphism(Cardiovascular Pharmacology, Basic/Clinical 2 (H) : OJ39)(Oral Presentation (Japanese))
- Elevation of Plasma Levels of ACTH and Aldosterone in Patients with Essential Hypertension; Possible Roles in Low Renin Hypertension
- Activation of Proopiomelanocortin Gene Expression in Failing Human Heart
- Possible Cardioprotective Effect of Dehydroepiandrosterone Produced in the Human Heart
- Aldosterone Induces Angiotensin-Converting-Enzyme Gene Expression through Both Mineralcorticoid -and Glucocorticoid- Receptors in Cultured Neonatal Rat Cardiocytes
- Inhibitory Effect of Natriuretic Peptides on Aldosterone Synthase Gene Expression in Cultured Neonatal Rat Cardiocytes
- Plasma B-Type Natriuretic Peptide as a Prognostic Marker of Myocardial Infarction : Ten Years Follow-Up Study
- Increased Risk of Coronary Spasm Due to the Combination of T^ to C Mutation in the eNOS Gene and Smoking
- Increased risk of coronary spasm due to the combination of-786T to C mutation in eNOS gene and smoking
- Detection of CYP17 gene expression and the regulatory mechanisms in human vascular smooth muscle cells
- Proopiomelanocortin (POMC) gene expression in the human autopsy heart
- Aldosterone is Produced From the Ventricles in Patients With Essential Hypertension
- Production of dehydroepiandrosterone (DHEA) in the human heart : Comparison between control subjects and heart failure patients
- Adrenocorticotropin (ACTH) Production in the Hypertensive Human Heart
- Pharmacokinetic Study of β-Methyldigoxin by Enzyme Immunoassay Using a Novel Specific Antiserum in Rats
- Establishment of Enzyme Immunoassay for Measuring β-Methyldigoxin Levels in Human Serum by Specific Antiserum(Analytical Biochemistry)
- Detection of Aldosterone Synthase Gene Expression in the Failing Human Ventricle : Quantitative Analysis Using a Newly Modified Real-Time RT-PCR
- Liquid chromatographic determination of 1-adamantanamine and 2-adamantanamine in human plasma after pre-column derivatization with o-phthalaldehyde and 1-thio-β-D-glucose
- Detection of CYP17 gene expression and dehydroepiandrosterone production in the human heart