Establishment of Enzyme Immunoassay for Measuring β-Methyldigoxin Levels in Human Serum by Specific Antiserum(Analytical Biochemistry)
スポンサーリンク
概要
- 論文の詳細を見る
We investigated the specificity of obtained antisera to β-methyldigoxin by the enzyme immunoassay. Three types of hapten-bovine serum albumin (BSA) conjugates were synthesized to obtain high specific antisera to β-methyldigoxin. The haptens were linked to the carrier protein through hemisuccinate at C-3' and C-3" positions in the digitoxose chain and at C-12 position in the aglycone. Anti-β-methyldigoxin 3'-hemisuccinate-BSA anti-serum showed a low detection limit (0.2 ng/ml) and possessed high specificity for β-methyldigoxin, exhibiting low cross-reactions with digoxigenin bisdigitoxoside (8.3%), dihydrodigoxin (4.8%), digitoxin (1.5%), and digoxigenin monodigitoxoside (0.95%), except for cross-reaction with digoxin (43%). Compared with commercial anti-digoxin antiserum, clinically used to monitor β-methyldigoxin concentration in human serum, cross-reaction data of anti-β-methyldigoxin 3'-hemisuccinate-BSA antiserum showed higher specificity for β-methyldigoxin. The intra-assay and inter-assay variations using this antiserum were less than 6.9% and 8.1%, respectively. The recovery tests were good, within the range of 96.2-104.3%. Phenyl boric acid (PBA) column treatment was effective to rapidly and selectively separate β-methyldigoxin from the mixture of β-methyldigoxin and its metabolites in human serum. The recovery tests of β-methyldigoxin with PBA column in human serum were about 110% and interference of metabolites of β-methyldigoxin was negligible. These results suggest that anti-β-methyldigoxin 3'-hemisuccinate-BSA antiserum and PBA column treatment are useful to more precisely monitor the unchanged type of β-methyldigoxin concentration in human serum.
- 公益社団法人日本薬学会の論文
- 2002-10-01
著者
-
HIGASHI Yasuhiko
Department of Analytical Chemistry, Faculty of Pharmaceutical Sciences, Hokuriku University
-
FUJII Youichi
Department of Analytical Chemistry, Faculty of Pharmaceutical Sciences, Hokuriku University
-
Fujii Y
Department Of Analytical Chemistry Faculty Of Pharmaceutical Sciences Hokuriku University
-
Fujii Youichi
Department Of Analytical Chemistry Faculty Of Pharmaceutical Sciences Hokuriku University
-
Watanabe Nobuaki
Drug Metabolism And Pharmacokinetics Research Laboratories Daiichi-sankyo Co. Ltd.
-
Watanabe N
Pharmacokinetics And Drug Delivery Research Laboratories Sankyo Co. Ltd.
-
Watanabe Naomi
Department Of Analytical Chemistry Faculty Of Pharmaceutical Sciences Hokuriku University
-
Higashi Yasuhiko
Department Of Analytical Chemistry Faculty Of Pharmaceutical Sciences Hokuriku University
-
Higashi Y
Department Of Analytical Chemistry Faculty Of Pharmaceutical Sciences Hokuriku University
-
SASAKI Toshiyuki
Department of Analytical Chemistry, Faculty of Pharmaceutical Sciences, Hokuriku University
-
Fujii Youichi
Fac. Of Pharmaceutical Sciences Hokuriku Univ.
-
Sasaki Toshiyuki
Department Of Analytical Chemistry Faculty Of Pharmaceutical Sciences Hokuriku University
-
Watanabe Nobuaki
Drug Metabolism and Pharmacokinetics Research Laboratories, Daiichi-Sankyo Co., Ltd.
関連論文
- Sensitive Determination of 4-(4-Bromophenyl)-4-hydroxypiperidine, a Metabolite of Bromperidol, in Rat Plasma by HPLC with Fluorescence Detection after Pre-column Derivatization Using 4-Fluoro-7-nitro-2,1,3-benzoxadiazole(Analytical Biochemistry)
- Substrate Deacylation Mechanism of Serine beta-lactamases
- Hydrolysis of Synthetic Substrate, L-Pyroglutamyl p-Nitroanilide is Catalyzed Solely by Pyroglutamyl Aminopeptidase I in Rat Liver Cytosol(Biochemistry/Molecular Biology)
- Pharmacokinetic Study of β-Methyldigoxin by Enzyme Immunoassay Using a Novel Specific Antiserum in Rats
- Establishment of Enzyme Immunoassay for Measuring β-Methyldigoxin Levels in Human Serum by Specific Antiserum(Analytical Biochemistry)
- Successful Treatment of Progressive Henoch-Schonlein Purpura Nephritis with Tonsillectomy and Steroid Pulse Therapy
- Catalytic Mechanism of Class A β-Lactamase. I. The Role of Glu166 and Ser130 in the Deacylation Reaction
- Functional Analysis of Aldehyde Oxidase Using Expressed Chimeric Enzyme between Monkey and Rat(Molecular and Cell Biology)
- Properties of 130 kDa Subunit of Monkey Aldehyde Oxidase(Molecular and Cell Biology)
- Cloning, Expression, and Characterization of Male Cynomolgus Monkey Liver Aldehyde Oxidase(Biochemistry)
- Reversible Pituitary Dysfunction in a Patient with Cushing's Syndrome Discovered as Adrenal Incidentaloma
- Identification of Rat Liver Aldehyde Oxidase across Species by Accurate Peptide-Mass Fingerprinting and Sequence-Tagging with Fourier Transform Ion Cyclotron Resonance Mass Spectrometry (プロテオーム)
- Properties of Novel Anti-digoxin Antisera in Radioimmunoassay Using Homologous and Site Heterologous Tritium-Labeled Antigens Involving a [^3H]-Leucine Moiety(Analytical Biochemistry)
- Liquid chromatographic determination of 1-adamantanamine and 2-adamantanamine in human plasma after pre-column derivatization with o-phthalaldehyde and 1-thio-β-D-glucose
- Functional Analysis of Aldehyde Oxidase Using Expressed Chimeric Enzyme between Monkey and Rat
- Adsorbing-particle flotation of Hg2+ by combined use of charcoal, polyacrylamide, and cationic surfactant.