Mapping Major Replication Origins on the Rice Plastid DNA
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概要
- 論文の詳細を見る
To maintain and to differentiate into various plastid lineages, replication of the plastid DNA (ptDNA) and division of the plastid must take place. However, replication initiation of the ptDNA has been less understood. The present study describes identification of the initiation region (origin) of ptDNA replication in the rice cultured cells. RNA-primed newly replicated DNA strands pulse-labeled with bromodeoxyuridine were isolated and size-fractionated. Locations of these nascent strands on the ptDNA determined the two major origin regions around the 3 region of each 23S rDNA in the inverted repeats (IRA and IRB). Two-dimensional agarose gel electrophoresis of the replication intermediates suggested that replication from each origin proceeds bidirectionally. This contrasted to replication by the double D-loop mechanism.
- 日本植物細胞分子生物学会の論文
- 2002-03-01
著者
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SATO Tadashi
Department of Environmental Science, Faculty of Science, Niigata University
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Saitoh Yasushi
United Graduate School Of Agricultural Sciences Iwate Univ.
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Tsutsumi Ken-ichi
United Graduate School of Agricultural Sciences, Iwate University
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Tsutsumi Ken-ichi
United Graduate School Of Agricultural Sciences Iwate Univ.
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Saitoh Yasushi
United Graduate School Of Agricultural Sciences Iwate University
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Tsutsumi Ken-ichi
United Graduate School Of Agricultural Sciences Iwate University
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Hidaka Soh
Department of Crop Breeding. Tohoku Nationa!Agricultural Experiment Station
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WANG Ying
United Graduate School of Agricultural Sciences, Iwate University
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TAMURA Kohya
Cryobiosystem Research Center, Iwate University
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Hidaka Soh
Department Of Crop Breeding National Agricultural Research Center For Tohoku Region
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Tamura Kohya
Cryobiosystem Research Center Iwate University
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Sato Tadashi
Department Of Environmental Science Faculty Of Science Niigata University
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Wang Ying
United Graduate School Of Agricultural Sciences Iwate University
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Sato Tadashi
Department Of Biochemistry Research Laboratory Of Applied Biochemistry Tanabe Seiyaku Co. Ltd.
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Sato Tadashi
Department Of Ecology And Evolutionary Biology Graduate School Of Life Science Tohoku University
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Sato Tadashi
Department Of Applied Chemistry Faculty Of Science And Engineering Waseda Uiversity
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