Distribution of the Extracellular Free Ca^<2+> in the Periodontal Ligament and Periosteum during Experimental Tooth Movement Using Fluorescent Free Ca^<2+> Indicator "Fluo-3"
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概要
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Distribution of extracellular free Ca2+ was examined in the rat periodontal ligament and periosteum of alveolar bone during tooth movement, using fluo-3. To stimulate bone formation and osteoclastic bone resorption, upper molars of adult rats were moved lingually for 7 days. After careful removal of the chestal ribs, fluo-3 was injected into the left ventricle of throbbing hearts, immediately after clamping the descending aortae with forceps. 90sec after the injection, the upper jaw was excised out rapidly and immersed in liquid nitrogen. 5μm unfixed undecalcified frozen sections from the frozen tissues were prepared for fluorescent microscopy and also electron microprobe analysis (EPMA). Green fluorescence was emitted from the whole periodontal space and pulp cavity. The pattern of bone formation in the periodontal ligament was quite different from that in the periosteum. A considerable amount of osteoid formed in the buccal periodontal ligament (tension side) and also in the periosteum of lingual alveolar bone (compensative bone apposition). The fluorescence emitted from the osteoid was more intense than that from the periodontal ligament or subperiosteal tissue. EPMA data also showed a higher peak of Ca in the osteoid than in the periodontal ligament or subperiosteal tissues. In addition, a narrow linear green fluorescence was emitted from the interface between the osteoclasts and the bone in the lingual periodontal ligament (pressure side). These results suggest that osteoid contains more free Ca2+ than periodontal ligament and subperiosteal tissue.
- 日本組織細胞化学会の論文
- 1995-08-01
著者
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Kuwahara Y
Tsurumi Univ. Yokohama Jpn
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Kuwahara Yosuke
Department Of Orthodontics School Of Dental Medicine Tsurumi University
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Nakamura Yoshiki
Department Of Neurosurgery National Hospital Organization Tokyo Medical Center
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Tanaka Takashi
Department Of Internal Medicine Fukui Cardiovascular Center
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NODA Kouji
Department of Orthodonitics, School of Denatal Medicine, Tsurumi University
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Noda Kouji
Department Of Orthodonitics School Of Denatal Medicine Tsurumi University
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Nakamura Yoshiki
Department Of Built Environment Interdisciplinary Graduate School Of Science And Engineering Tokyo I
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Nakamura Yoshiki
Department Of Orthodonitics School Of Denatal Medicine Tsurumi University
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Tanaka Takashi
Department Of Central Clinical Laboratory Teikyo University Hospital
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Tanaka Takashi
Department Of Orthodonitics School Of Denatal Medicine Tsurumi University
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Kuwahara Yosuke
Department of Materials Science and Engineering, Meijo University, Nagoya 468-8502, Japan
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