蛍光誘導体化による食用油脂中のステロールのHPLC分析

概要

論文の詳細を見る
A simple and highly sensitive high-performance liquid chromatographic method for determining sterols in edible oils and fats is presented. Sterols were isolated from a few miligrams of sample by saponification or thin layer chromatography. They were subsequently converted into the corresponding fluorescent derivatives by heating with 9-anthroylnitrile at 100°C for 30 min in the presence of triethylamine. The derivatives were separated on a reversed phase column (Senshu pak ODS-1250-Y) with an isocratic elution using acetonitrile-dichloromethane (75/25, vol/vol). The detection limit for cholesterol was about 80 pg. The present method was shown applicable to the determination of total, free and acylated sterols in edible oils and fats with satisfactory reliability, sensitivity and handling.
社団法人日本油化学会の論文
1992-04-20