Identification and Characterization of the Hematopoietic Cell-Specific Enhancer-Like Element of the Mouse Hex Gene
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概要
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Hex is one of the homeobox genes suggested to be important for hematopoietic cell differentiation. However, its biological function and mechanism of transcriptional regulation in hematopoietic cells remain elusive. We have identified the regulatory region necessary for transcription of the mouse Hex gene in K 562 leukemia cells through transient reporter assays involving various deletion mutants. This region, comprising +775 to +1177 in the first intron, had enhancer-like properties and showed high activity in other hematopoietic cell lines such as U 937, HEL, and RAW 264.7, but little activity in other Hex-expressing cell lines such as MH1C1 and H4IIE hepatoma cells, suggesting that this region functions as a hematopoietic cell-specific enhancerlike element. Binding site mutation of hematopoietic transcription factors, such as GATAs and c-Myb present in the enhancer-like element, significantly decreased the luciferase reporter gene expression in K562 cells. Electrophoretic mobility shift assays showed that GATA-1, GATA-2, or c-Myb actually binds to three of these putative binding sites, and also suggested that several unidentified factors might interact with the enhancer-like element. Overexpression of GATA-1, GATA-2, or c-Myb stimu-lated the enhancer-like activity via these three binding sites. Thus, we conclude that Hex expression in hematopoietic cells is mainly regulated by GATA-1, GATA-2, and c-Myb via this intronic enhancer-like element.
- 社団法人 日本生化学会の論文
著者
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Keng Vincent
Department Of Applied Molecular Biosciences Nagoya University Graduate School Of Bioagricultural Sci
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Yamamoto Taichi
Department Of Applied Biological Science Tokyo University Of Science
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SATO Ayuko
Department of Applied Molecular Biosciences, Graduate School of Bioagricultural Sciences, Nagoya Uni
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Ban Tomoko
Department Of Applied Molecular Biosciences Graduate School Of Bioagricultural Sciences Nagoya Unive
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Satoh Shin-ichi
Department Of Applied Molecular Biosciences Graduate School Of Bioagricultural Sciences Nagoya Unive
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Kasamatsu Shinya
Department Of Applied Molecular Biosciences Graduate School Of Bioagricultural Sciences Nagoya Unive
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Tanaka Hironori
Department Of Applied Molecular Biosciences Graduate School Of Bioagricultural Sciences Nagoya Unive
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Yamada Kazuya
Department of Biochemistry Fukui Medical University
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Noguchit Tamio
Department of Applied Molecular Biosciences, Graduate School of Bioagricultural Sciences, Nagoya University
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