Insulin Stimulates Expression of the Pyruvate Kinase M Gene in 3T3-L1 Adipocytes(Biochemistry & Molecular Biology)
スポンサーリンク
概要
- 論文の詳細を見る
M_2-type pyruvate kinase (M_2-PK) mRNA is produced from the PKM gene by an alternative RNA splicing in adipocytes. We found that insulin increased the level of M_2-PK mRNA in 3T3-L1 adipocytes in both time- and dose-dependent manners. This induction did not require the presence of glucose or glucosamine in the medium. The insulin effect was blocked by pharmacological inhibitors of insulin signaling pathways such as wortmannin, an inhibitor of phosphatidylinositol 3-kinase (PI3K), and PD98059, an inhibitor of mitogen-activated protein kinase (MAPK) kinase. A stable reporter expression assay showed that the promoter activity of an about 2.2-kb 5'-flanking region of the rat PKM gene was stimulated by insulin, but the extents of these stimulations were lower than those of the mRNA stimulation. Thus, we suggest that insulin increases the level of M_2-PK mRNA in adipocytes by acting at transcriptional and post-transcriptional levels through signaling path-ways involving both PI3K and MAPK kinase.
- 社団法人日本農芸化学会の論文
- 2003-06-23
著者
-
Yamada K
Department Of Biochemistry Fukui Medical University And Crest Japan Science And Technology
-
Yamada Kazuya
Department Of Fixed Prosthodontics Nihon University School Of Dentistry
-
Keng Vincent
Department Of Applied Molecular Biosciences Nagoya University Graduate School Of Bioagricultural Sci
-
Noguchi Tamio
Department Of Applied Molecular Biosciences Nagoya University Graduate School Of Bioagricultural Sci
-
ASAI Yuuki
Department of Applied Molecular Biosciences, Nagoya University Graduate School of Bioagricultural Sc
-
WATANABE Toyoaki
Department of Applied Molecular Biosciences, Nagoya University Graduate School of Bioagricultural Sc
-
Watanabe Toyoaki
Department Of Applied Molecular Biosciences Nagoya University Graduate School Of Bioagricultural Sci
-
Asai Yuuki
Department Of Applied Molecular Biosciences Nagoya University Graduate School Of Bioagricultural Sci
-
Yamada Kazuya
Department of Biochemistry Fukui Medical University
関連論文
- Effect of single-liquid priming agents on adhesive bonding to aluminum oxide of a methacrylic resin.
- EXPRESSION ANALYSIS OF EPIREGULIN AND AMPHIREGULIN IN THE RAT OVARY
- THE GENE EXPRESSION AND TRANSCRIPTIONAI ACTIVITY OF NUCLEAR RECEPTOR COACTIVATOR P120 IN RAT OVARY
- TRANSCRIPTIONAL REGULATION OF EPIREGULIN GENE IN THE RAT OVARY
- EARLY GROWTH RESPONSE GENE-1 (EGR-1) REGULATES THE EXPRESSION OF THE RAT LUTEINIZING HORMONE RECEPTOR GENE
- Sp Family Members Stimulate Transcription of the Hex Gene via Interactions with GC Boxes
- Genomic Organization and Promoter Analysis of a Mouse homeobox Gene, Hex
- Identification of the Transactivating Region of the Homeodomain Protein, Hex
- Identification and Characterization of the Hematopoietic Cell-Specific Enhancer-Like Element of the Mouse Hex Gene
- Insulin Stimulates Expression of the Pyruvate Kinase M Gene in 3T3-L1 Adipocytes(Biochemistry & Molecular Biology)
- Relationship between the Concentrations of Glycolytic Intermediates and Expression of the L-Type Pyruvate Kinase Gene in Cultured Hepatocytes
- High Hole Mobility in GaAsxBi[x] Alloys
- The Effect of a High-Protein Diet on Cystathionine .BETA.-Synthase Activity and Its Transcript Levels in Rat Liver.
- The Role of the Autonomic Nervous System in the Thermic Effects of Protein and Carbohydrates in Rats.
- Effect of Cysteine on Expression of Cystathionine .BETA.-Synthase in the Rat Liver.
- Identification of the Transactivating Region of the Homeodomain Protein, Hex
- Identification and Characterization of the Hematopoietic Cell-Specific Enhancer-Like Element of the Mouse Hex Gene
- Sp Family Members Stimulate Transcription of the Hex Gene via Interactions with GC Boxes.