Exposure to High Doses of Lipopolysaccharide during Ovalbumin Sensitization Prevents the Development of Allergic Th2 Responses to a Dietary Antigen
スポンサーリンク
概要
- 論文の詳細を見る
Food allergies are driven by aberrant T helper (Th) 2 cells. Lipopolysaccharide (LPS) influences the development of Th2-mediated diseases, but its role in food allergy and tolerance remains unclear. To address this issue, we established mouse models presenting allergic or tolerant responses to ovalbumin (OVA). Mice sensitized with crude OVA developed Th2 responses including acute diarrhea, increases in serum OVA-specific IgE, dominant production of serum OVA-specific IgG1, increases in Th2-type cytokines and proliferation of mast cells in duodenal and colonic tissues. Sensitization of mice with crude OVA and LPS abrogated Th2-type responses observed in allergic mice. The level of OVA-specific proliferation in mesenteric lymph node CD4+ T cells was comparable in allergic and tolerant mice, indicating that the tolerance is not caused by anergy and apoptosis of antigen-primed T cells. Expression of Th1- and Th2-type cytokines was suppressed in whole spleen cells and/or purified spleen CD4+ T cells of tolerant mice, indicating that the tolerance was not caused by the shift from Th2 to Th1. On the other hand, interleukin (IL)-10, a regulatory cytokine produced by regulatory T cells, was upregulated in whole spleen cells and purified spleen CD4+ T cells of tolerant mice. Furthermore, spleen CD4+ T cells from tolerant mice suppressed the growth of CD4+ T cells from DO11.10 mice in co-culture. These results indicate that tolerance is induced in allergic mice by simultaneous exposure to LPS during sensitization with OVA and that a population of T cells producing IL-10 plays an important role in the tolerance induction.
- 日本毒性病理学会の論文
著者
-
Torii Ikuko
Department Of Pathology First Unit Shimane Medical University
-
Shimizu Shigeki
Department Of Chemistry Faculty Of Science University Of Kyoto
-
SATO Ayuko
Department of Applied Molecular Biosciences, Graduate School of Bioagricultural Sciences, Nagoya Uni
-
Kudo Tomoo
Department Of Pathology Nippon Dental University School Of Life Dentistry At Tokyo
-
SHINOHARA Yoshiyasu
Department of Gastroenterology, Yokohama City University Graduate School of Medicine
-
Daimon Takashi
Department Of Biostatics Hyogo Medical College
-
TSUJIMURA Tohru
Department of Molecular Pathology, Hyogo College of Medicine, 1-1 Mukogawa-cho, Nishinomiya, Hyogo 663-8501, Japan
-
DAIMON Takashi
Department of Biostatistics, Hyogo College of Medicine, 1-1 Mukogawa-cho, Nishinomiya, Hyogo 663-8501, Japan
関連論文
- Crucial role of impaired Kupffer cell phagocytosis on the decreased Sonazoid-enhanced echogenicity in a liver of a nonalchoholic steatohepatitis rat model
- Effect of arbekacin on a methicillin-resistance Staphylococcus aureus-induced biofilm in a rat model
- TAK1 is indispensable for development of T cells and prevention of colitis by the generation of regulatory T cells
- Chemoprevention of spontaneous development of hepatocellular carcinomas in fatty liver Shionogi mice by a cyclooxygenase-2 inhibitor
- A Case of Acute Interstitial Pneumonia Indistinguishable from Bronchiolitis Obliterans Organizing Pneumonia/Cryptogenic Organizing Pneumonaia: High-Resolution CT Findings and Pathologic Correlation
- Application of Fluorescence Polarization Immunoassay for Determination of Methotrexate-Polyglutamates in Rheumatoid Arthritis Patients
- An Electron Diffraction Study and a Theory of the Transformation from γ-Fe_2O_3 to α-Fe_2O_3
- Fos Plays No Role in Apoptosis of Epithelia in the Mouse Male Accessory Sex Organs and Uterus
- Castration Induces Apoptosis in the Mouse Epididymis during Postnatal Development
- A human B-lineage dendritic cell line, HBM-Noda and its potential role in human T-cell leukemia/lymphoma virus type I infection
- Cytological Characteristics of Four Longterm Cultured Human Gastric Adenocarcinoma Cell Lines
- Effect of TPA or retinoic acid on the production of carcinoembryonic antigen by human gastric carcinoma cell lines
- Identification of the Transactivating Region of the Homeodomain Protein, Hex
- Identification and Characterization of the Hematopoietic Cell-Specific Enhancer-Like Element of the Mouse Hex Gene
- P-14 Enzyme-and Immuno-histochemical Analysis of Human Hematopoietic Cell Lines with Endocytotic Activity
- Three-dimensional visualization and quantification for the growth and invasion of oral squamous cell carcinoma
- Establishment of a cell line from a Japanese patient useful for generating an in vivo model of malignant pleural mesothelioma
- Alveolar Adenoma of the Lung : A Case Report
- Semaphorin 4D, a lymphocyte semaphorin, enhances tumor cell motility through binding its receptor, plexinB1, in pancreatic cancer
- Predictor of Early Mortality for Severe Heart Failure Patients With Left Ventricular Assist Device Implantation : Significance of INTERMACS Level and Renal Function
- Practical approaches to diagnose and treat for T0 malignant pleural mesothelioma : a proposal for diagnostic total parietal pleurectomy
- Kikuchi-Fujimoto Disease Mimicking Tuberculous Lymphadenitis
- Pathological and molecular biological approaches to early mesothelioma
- Urgent Management of Rapid Heart Rate in Patients With Atrial Fibrillation/Flutter and Left Ventricular Dysfunction:– Comparison of the Ultra-Short-Acting β1-Selective Blocker Landiolol With Digoxin (J-Land Study) –
- Urgent Management of Rapid Heart Rate in Patients With Atrial Fibrillation/Flutter and Left Ventricular Dysfunction : Comparison of the Ultra-Short-Acting β1-Selective Blocker Landiolol With Digoxin (J-Land Study)
- Identification of the Transactivating Region of the Homeodomain Protein, Hex
- Identification and Characterization of the Hematopoietic Cell-Specific Enhancer-Like Element of the Mouse Hex Gene
- Exposure to High Doses of Lipopolysaccharide during Ovalbumin Sensitization Prevents the Development of Allergic Th2 Responses to a Dietary Antigen