Functional Analysis of cDNAs for Two Types of Human Heme Oxygenase and Evidence for Their Separate Regulation.
スポンサーリンク
概要
- 論文の詳細を見る
We cloned a cDNA coding for a putative human heme oxygenase isozyme, designated type 2 (HO-2), and analyzed its function by transient expression assays. HeLa cells transfected with either HO-2 cDNAs or a cDNA coding for authentic heme oxygenase (HO-1) expressed the activity of heme oxygenase, although no activity was detected in the mock transfected cells. Using specific anti-HO-1 antibody, we showed that expression of a HO-1 cDNA resulted in the increase in its protein levels, but HO-1 protein was not detectable in the cells expressing HO-2 cDNAs. We thus confirmed the functional identity of HO-1 and HO-2. Then, we analyzed their expression in an erythroid cell line, YN-1-0-A. Treatment with hemin or by heat shock (42°C) led to a remarkable increase in the HO-1 mRNA levels, while HO-2 mRNA expression was not induced at all, suggesting that they are under separate regulation.
- 社団法人 日本生化学会の論文
著者
-
YOSHIZAWA Miki
Department of Molecular Biology and Applied Physiology Tohoku University School of Medicine
-
Shibahara Shigeki
Department Of Applied Physiology And Molecular Biology Tohoku University School Of Medicine
-
Takeda Kazuhisa
Department Of Gastroenterological Surgery Yokohama City University
-
Suzuki Hiroyuki
Department Of Animal Science Faculty Of Agriculture Hokkaido University
-
Meguro Kuniaki
Second Department of Internal Medicine, Tohoku University School of Medicine
-
Endo Kazuyasu
Second Department of Internal Medicine, Tohoku University School of Medicine
関連論文
- High expression of Pirh2, an E3 ligase for p27, is associated with low expression of p27 and poor prognosis in head and neck cancers
- Symmetry Breaking in a Frustrated Heisenberg Spin System, ZnCr_2O_4 : I. Magnetic Measurements(Condensed matter: electronic structure and electrical, magnetic, and optical properties)
- Conversion from Total Cavopulmonary Shunt to Fontan Circulation : Improved Cyanosis with an 11-Year Interval
- BRM cocktail treatment using PSK and OK432 significantly up-regulates the migration activity in human dendritic cells without losing effective CTL induction
- Augmented Chemosensitivity in Black-Eyed White Mitf^ Mice, Lacking Melanocytes
- Germ Cell-Specific Expression of Microphthalmia-Associated Transcription Factor mRNA in Mouse Testis
- Decreased Expression of Adrenomedullin during Adipocyte-Differentiation of 3T3-L1 Cells
- Molecular Cloning of cDNA Encoding a Novel Microphthalmia-Associated Transcription Factor Isoform with a Distinct Amino-Terminus
- Role of Neurofibromin in Modulation of Expression of the Tyroshinase-Related Protein 2 Gene
- Differential Tissue-Specific Expression of Neurofibromin Isoform mRNAs in Rat^1
- Evidence for the Presence of Two Amino-Terminal Isoforms of Neurofibromin, a Gene Product Responsible for Neurofibromatosis Type 1
- A Prolyl-hydroxylase Inhibitor, Ethyl-3,4-dihydroxybenzoate, Induces Haem Oxygenase-1 Expression in Human Cells Through a Mechanism Independent of Hypoxia-inducible Factor-1α
- Functional Difference of the SOX10 Mutant Proteins Responsible for the Phenotypic Variability in Auditory-Pigmentary Disorders
- Crossover from Magnetic to Nonmagnetic Ground State in the Kondo Alloy System Ce (Ni_Pd_x) Sn
- Nucleotide Alteration of Retinoblastoma Protein-Interacting Zinc Finger Gene, RIZ, in Human Leukemia
- Magnetic Properties of New Ternary Rare Earth Compounds RPt (Pd) Sb
- MCH-01 DEVELOPMENT OF A NOVEL METHOD FOR STRETCHING DNA FIBERS ON MICROBRIDGES FABRICATED BY SINGLE-MASK INCLINED UV LITHOGRAPHY(Micro/Nanomechatronics I,Technical Program of Oral Presentations)
- Strong Anisotropy of Transport Properties in Metamagnetic CePtAs
- Long-term rectal temperature measurements in a patient with menstrual-associated sleep disorder
- Effects of nocturnal bright light on saliva melatonin, core body temperature and sleep propensity rhythms in human subjects
- Reservoir Competence of the Vole, Clethrionomys rufocanus bedfordiae, for Borrelia garinii or Borrelia afzelii
- COMMON PATHOGENIC MECHANISM IN DEVELOPMENT PROGRESSION OF LIVER INJURY CAUSED BY NON-ALCOHOLIC OR ALCOHOLIC STEATOHEPATITIS
- Transcriptional Activation of the Melanocyte-Specific Genes by the Human Homolog of the Mouse Microphthalmia Protein
- Tristetraprolin (TTP) gene polymorphisms in patients with rheumatoid arthritis and healthy individuals
- Giant mesenchymal hamartoma of the liver in an adult
- Porcelain gallbladder complicated with pancreas divisum
- Purification and Some Properties of NADH-Dependent Sulfite Reductase from Escherichia coli Harboring Plasmid pTHS1,Which Has the S-Adenosyl-L-Methionine : Uroporphyrinogen III Methyltransferase Gene of Thiobacillus ferrooxidans
- A DNA Region That Complements on Escherichia coli cysG Mutation in Thiobacillus ferrooxidans
- NADH-dependent Sulfite Reductase Activity in the Periplasmic Space of Thiobacillus ferrooxidans
- Inhibition of Sulfur Use by Sulfite Ion in Thiobacillus ferrooxidans(Microbiology & Fermentation Industry)
- Purificaion and Some Properties of a Hydrogen Sulfide-binding Protein That Is Involved in Sulfur Oxidation of Thiobacillus ferrooxidans(Microbiology & Fermentation Industry)
- Diurnal fluctuation of time perception under 30-h sustained wakefulness
- Time estimation during nocturnal sleep in human subjects
- Effects of small dose of brotizolam on P300
- Possible Involvement of 3-Dehydroteasterone in the Conversion of Teasterone to Typhasterol in Cultured Cells of Catharanthus roseus
- Reduced Atherosclerotic Lesion Formation and Chronic Hypoxemia in Heme Oxygenase-2 Knockout Mice(Atherosclerosis, Basic 1 (IHD), The 69th Annual Scientific Meeting of the Japanese Circulation Society)
- Mice with Blunted Hypoxic Ventilatory Response are Susceptible to Respiratory Disturbance during Hypoxia
- Serum levels of neutrophil activation cytokines in Kawasaki disease
- Evaluation of Pretreatment with Pleurotus ostreatus for Enzymatic Hydrolysis of Rice Straw(ENVIRONMENTAL BIOTECHNOLOGY)
- Estimation of Collector Current Spreading in InGaAs SHBT Having 75-nm-Thick Collector
- First NMR Observation of Valence Fluctuation in Rare-Earth Compounds : ^Se NMR Studies of Temperature-Activated Valence Fluctuation in Sm_3Se_4
- Two nap sleep test : An easy objective sleepiness test
- Possible Implications of the Induction of Human Heme Oxygenase-1 by Nitric Oxide Donors^1
- Evidence-Based Infection Control in Thoracic Surgery
- Identification of Brassinolide, Castasterone, Typhasterol, and Teasterone from the Pollen of Lilium elegans
- Circadian fluctuation of time perception in healthy human subjects
- Comparison of OspA Serotypes for Borrelia burgdorferi Sensu Lato from Japan, Europe and North America
- Presence of Common Antigenic Epitope in Outer Surface Protein(Osp)A and OspB of Japanese Isolates Identified as Borrelia garinii
- REGULATION OF TGF-β SIGNALING AND ITS ROLES IN PROGRESSION OF TUMORS
- Regulation of TGF-β signaling and its roles in progression of tumors
- Neuroendocrine Functions of Melanocytes : Beyond the Skin-Deep Melanin Maker
- Strategies for Treatment of Acute Aortic Dissection with Involvement of Sinus of Valsalva
- Biosynthesis of Brassinosteroids in Seedlings of Catharanthus roseus, Nicotiana tabacum, and Oryza sativa
- Changes in cuticle of scalp hair in mild acquired zinc deficiency: A study using scanning electron microscopy
- Physiological Implication of Induction of Heme Oxygenase-1 Gene Expression
- Heme-Regulated Transcription Factor Bach1
- Lymphoepithelial Cyst in the Sublingual Region : Report of a case and review of literature
- Neoplasms in three patients following Kawasaki disease
- Transforming growth factor-β signaling is differentially inhibited by Smad2D450E and Smad3D407E
- Self-Injection-Locked, Narrow-Linewidth, Flashlamp-Pumped Ti:Al_2O_3 Laser
- A Case of Terminal Deletion of the Long Arm of Chromosome 4, del(4)(q33→ter)
- Non-Kramer's Doublet Ground State in PrPtBi
- Clinical Characteristics of Patients With Kawasaki Disease and Levels of Peripheral Endothelial Progenitor Cells and Blood Monocyte Subpopulations
- Construction of a System that Simultaneously Evaluates CYP1A1 and CYP1A2 Induction in a Stable Human-derived Cell Line using a Dual Reporter Plasmid
- Time estimation during sleep relates to the amount of slow wave sleep in humans
- Thermal Dissociation of Disilenes into Silylenes
- Estimation of collector current spreading in InGaAs SHBT having 75-nm-thick collector
- Estimation of collector current spreading in InGaAs SHBT having 75-nm-thick collector
- Pruritic Papular Eruptions and Candidiasis Due to HIV Infection
- Acquired Reactive Perforating Collagenosis with IgA Nephropathy
- Hypoxemia induces expression of heme oxygenase-1 and heme oxygenase-2 proteins in the mouse myocardium
- Lipocalin-type prostaglandin D synthase as a regulator of the retinoic acid signalling in melanocytes
- A case of diaphragmatic clear cell carcinoma in a patient with a medical history of ovarian endometriosis
- Cerebrospinal Fluid Cytokines in Salmonella Urbana Encephalopathy
- Bullous Pemphigoid in an HB Virus Carrier : Interaction between Corticosteroids and HB Virus
- Edematous Changes in Mucosal Folds of the Internal Os of the Rabbit Cervix
- Lipid-rich Keratin Spherules Containing Cholesterol Crystals in an Epidermal Cyst : A Study Using Electron Microscopy
- ^Sn NMR Studies of the Heavy-Electron Compound U_3Au_3Sn_4
- Continuous Speech Recognition Based on General Factor Dependent Acoustic Models(Feature Extraction and Acoustic Medelings, Corpus-Based Speech Technologies)
- Successful treatment of refractory warts with topical vitamin D_3 derivative (maxacalcitol, 1α, 25-dihydroxy-22-oxacalcitriol) in 17 patients
- Coarse Columnar Structure of Transformation-Grown Ferrite in Pure Iron : On Wrought Iron and Sintered Iron
- Effects of Prostaglandin F2a on Egg Recovery from the Vagina and Egg Transport in Superovulated Rabbits
- Ciliation in Endometrial Epithelium of the Rabbit Following Ovariectomy
- Cervical Epithelium of the Rabbit Following Ovariectomy
- Morphological Changes of Uterine and Cervical Epithelium During Early Pregnancy in Rabbits
- T-4-3-3 Giant-swing motions of a 3-DOF Link Mechanism
- Nationwide survey and establishment of a clinical database for gastrointestinal surgery in Japan : Targeting integration of a cancer registration system and improving the outcome of cancer treatment
- EFFECTS OF LOPERAMIDE ON ACETYLCHOLINE AND PROSTAGLANDIN RELEASE FROM ISOLATED GUINEA PIG ILEUM
- The 2011 Tohoku Earthquake and Devastating Tsunami
- The Upcoming 90th Anniversary of the Tohoku Journal of Experimental Medicine
- The Heme Oxygenase Dilemma in Cellular Homeostasis: New Insights for the Feedback Regulation of Heme Catabolism
- Manifestations of Transthyretin-Related Familial Amyloidotic Polyneuropathy : Long-Term Follow-Up of Japanese Patients After Liver Transplantation
- Severe outflow block syndrome caused by compression by the swollen caudate lobe after living donor liver transplantation : report of a case
- Right hepatectomy with resection of caudate lobe and extrahepatic bile duct for hilar cholangiocarcinoma
- Emergency versus elective living-donor liver transplantation : a comparison of a single center analysis
- Coordinated Expression of 6-Phosphofructo-2-kinase/Fructose-2,6-bisphosphatase 4 and Heme Oxygenase 2 : Evidence for a Regulatory Link between Glycolysis and Heme Catabolism
- Germ Cell-Specific Expression of Microphthalmia-Associated Transcription Factor mRNA in Mouse Testis
- The effectiveness and appropriate management of abdominal drains in patients undergoing elective liver resection : a retrospective analysis and prospective case series
- Functional Analysis of cDNAs for Two Types of Human Heme Oxygenase and Evidence for Their Separate Regulation.
- Role of Neurofibromin in Modulation of Expression of the Tyrosinase-Related Protein 2 Gene.