Effects of [6]-Gingerol on Dedifferentiation of Salivary Acinar Cells
スポンサーリンク
概要
- 論文の詳細を見る
Salivary gland atrophy is an important problem in clinical dentistry since salivary glands produce and secrete saliva that creates and regulates the environment of the oral cavity. To clarify mechanisms of salivary gland dysfunction, we have established a system for primary culture of parotid acinar cells. We have found that the process of cell isolation induced the stress signal mediated by Src and p38 MAP kinases, which causes the alteration in expression patterns of various differentiation markers. Claudin-4 began to be expressed after the cell isolation and increased during the culture while the original acinar cells do not express claudin-4. In addition, the expression of salivary acinar markers such as amylase and aquaporin-5 were rapidly decreased during the culture, which implies the dedifferentiation of acinar cells. In this study, we examined the effects of [6]-gingerol,which is an ingredient of ginger, on the dedifferentiation of parotid acinar cells. We found that the increase of expression level of claudin-4 mRNA during the culture was suppressed by addition of [6]-gingerol. The effect was confirmed by immunoblot analysis. In addition,the decrease of amylase and aquaporin-5 during the culture was also suppressed by [6] -gingerol. These results suggest that [6]-gingerol has a protective effect against the cellular stresses that induce dysfunction of salivary glands.
著者
-
Narita Takanori
Department Of Biological Sciences Graduate School Of Science University Of Tokyo
-
Sugiya Hiroshi
Department Of Physiology And Research Institute Of Oral Science Nihon University School Of Dentistry
-
Yokoyama Megumi
Department Of Toxicology School Of Veterinary Medicine Rakuno-gakuen University
-
Fukumoto Masahiko
Department Of Comprehensive Clinical Dentistry Research Institute Of Oral Science Nihon University S
-
Okabayashi Ken
Department of Biology, Faculty of Science, Kobe University
-
HARUKI Mitsuru
Department of Chemical Biology and Applied Chemistry, College of Engineering, Nihon University
-
Katsumata-Kato Osamu
Departments of Physiology, Nihon University School of Dentistry at Matsudo, Matsudo,Chiba 271-8587, Japan
-
Yokoyama Megumi
Departments of Physiology, Nihon University School of Dentistry at Matsudo, Matsudo,Chiba 271-8587, Japan
-
Matsuki-Fukushima Miwako
Departments of Physiology, Nihon University School of Dentistry at Matsudo, Matsudo,Chiba 271-8587, Japan
-
Nagai-Fuse Megumi
Departments of Laboratory Medicine for Dentistry, Nihon University School of Dentistry at Matsudo, Matsudo,Chiba 271-8587, Japan
-
Fujita-Yoshigaki Junko
Departments of Physiology, Nihon University School of Dentistry at Matsudo, Matsudo,Chiba 271-8587, Japan
-
Matsuki-Fukushima Miwako
Department of Physiology, Nihon University School of Dentistry at Matsudo
-
Narita Takanori
Department of Veterinary Medicine, College of Bioresource Science, Nihon University, Fujisawa, Kanagawa 252-0880, Japan
-
Sugiya Hiroshi
Department of Veterinary Medicine, College of Bioresource Science, Nihon University, Fujisawa, Kanagawa 252-0880, Japan
関連論文
- Antifungal Activity of Itraconazole and Voriconazole against Clinical Isolates Obtained from Animals with Mycoses
- Endothelin-3 controls growth of colonic epithelial cells by mediating epithelial-mesenchymal interaction
- A Clinical and Histopathological Study of Guided Bone Regeneration Using Polytetrafluoroethylene Membrane in the Wound Resulting from Human Tooth Extraction
- Mutation in the abcb7 gene causes abnormal iron and fatty acid metabolism in developing medaka fish
- MEDAKA GENOME SEQUENCING AND THE VERTEBRATE GENOME EVOLUTION(Genetics,Abstracts of papers presented at the 76^ Annual Meeting of the Zoological Society of Japan)
- Draft Genome Sequence of Medaka (Orizias latipes) and Its Useful Applications(A Synthesis of the Molecular Phylogeny and the Comparative Genomics in Fishes,Symposium,PROCEEDING OF THE 76^ ANNUAL MEETING OF THE ZOOLOGICAL SOCIETY OF JAPAN)
- Medaka Genome Sequencing and the Comparative Genomics of Fishes(Comparative Genomics of Animals,Symposium,PROCEEDING OF THE 76^ ANNUAL MEETING OF THE ZOOLOGICAL SOCIETY OF JAPAN)
- Effect of the Scent from Grapefruit Oil on Salivary Secretion in Rats
- "P-20 Cytotoxicity of hemorrhagic snake venom on cultured endothelial cells.
- Gene Cloning and Characterization of Aldehyde Dehydrogenase from a Petroleum-Degrading Bacterium, Strain HD-1
- Diagnostic Problems of Early and Primary Lesions of Tuberculous Cervical Lymphadenitis
- GENETIC AND EMBRYOLOGICAL ANALYSIS OF LATE DEVELOPMENT OF THE LIVER USING MEDAKA MUTANTS(Developmental Biology,Abstracts of papers presented at the 76^ Annual Meeting of the Zoological Society of Japan)
- Characterization of Petroleum-Degrading Bacteria from Oil-Contaminated Sites in Vietnam
- In vitro Study of Collagen Coating of Titanium Implants for Initial Cell Attachment
- Isolation and Characterization of Xanthobacter polyaromaticivorans sp. nov. 127W That Degrades Polycyclic and Heterocyclic Aromatic Compounds under Extremely Low Oxygen Conditions
- Production and Characterization of Biosurfactants from Bacillus licheniformis F2.2(Microbiology & Fermentation Technology)
- 7. Transforming growth factor-b1 regulation of bone sialoprotein gene transcription.
- Importance of an N-Terminal Extension in Ribonuclease HII from Bacillus stearothermophilus for Substrate Binding
- Gene Cloning of an Alcohol Dehydrogenase from Thermophilic Alkane-Degrading Bacillus thermoleovorans B23
- Characterization of Ribonuclease HII from Escherichia coli Overproduced in a Soluble Form
- Ca^/Calmodulin-Dependent Nitric Oxide Synthase in the Rabbit Parotid Gland
- Identification of the Gene Encoding Esterase, a Homolog of Hormone-Sensitive Lipase, from an Oil-Degrading Bacterium, Strain HD-1
- Stabilities of Chimeras of Hyperthermophilic and Mesophilic Glycerol Kinases Constructed by DNA Shuffling
- Gene Cloning, Overproduction, and Characterization of Thermolabile Alkaline Phosphatase from a Psychrotrophic Bacterium
- Gene Cloning and Biochemical Characterizations of Thermostable Ribonuclease HIII from Bacillus stearothermophilus
- Cleavage of Various Peptides with Pitrilysin from Escherichia coli : Kinetic Analyses Using β-Endorphin and Its Derivatives
- Role of Repetitive Nine-Residue Sequence Motifs in Secretion, Enzymatic Activity, and Protein Conformation of a Family I.3 Lipase
- A Consideration of Papillary Squamous Cell Carcinoma of the Gingiva : A Case Study Involving the Immunohistochemistry of Proliferative Activity
- Analysis of Correlation between Caries Risk Factors to Establish an Effective Clinical Testing and Diagnostic System for Caries Risk
- Protein Kinase C Synergistically Stimulates Tumor Necrosis Factor-α-Induced Secretion of Urokinase-Type Plasminogen Activator in Human Dental Pulp Cells
- Crystallization and Preliminary X-Ray Diffraction Analysis of the Extracellular Domain of the Cell Surface Antigen CD38 Complexed with Ganglioside^1
- Isolation and Characterization of Long-Chain-Alkane Degrading Bacillus thermoleovorans from Deep Subterranean Petroleum Reservoirs
- Sphingosine Inhibits GTPγS-stimulated Inositol Trisphosphate Formation in Rat Parotid Acinar Cells
- Role of protein kinase C in cAMP-dependent exocytosis in parotid acinar cells
- Chloroplast Development in the Chloroplast Ribosome Deficient Mutant (y-1 ac-20) of Chlamydomonas reinhardtii
- Molecular Diversities of RNases H
- Reduced production of polymeric immunoglobulin receptor in murine dextran sodium sulfate-induced colitis
- Postnatal Lymphotoxin-.ALPHA.-Mediated Signals Reorganize Splenic Microarchitecture
- Effects of [6]-Gingerol on Dedifferentiation of Salivary Acinar Cells
- Frequency of Staphylococci in Oral Cavity of Healthy Medical Workers
- Evaluation of Buffering Capacity of Resting Saliva and Caries Prevalence for Caries Risk Assessment
- Origins of Nasal and Oral Staphylococci
- Associations among distributions of dental postgraduate residents, dentists and clinical training facilities in Japan
- Traditional Japanese Fermented Food Natto Enhances NK Cell Activity in Intestine
- Mandibular B-Cell Lymphoma in a 3-Year-Old Child: A Clinical Rarity
- Argininosuccinate Synthetase Activity in Parotid Glands
- New Primer Design for Identification of Oral rothia, Including R. aeria, Using Multiplex PCR
- Enhancement of the Enzymatic Activity of Escherichia coli Acetyl Esterase by a Double Mutation Obtained by Random Mutagenesis