Characteristics of Human Erythrocytes with Artific Ally-Increased ATP and/or 2, 3-Diphosphoglycerate Contents

概要

論文の詳細を見る
Incubation of fresh human erythrocytes with adenine and inosine in isotonic NaCI-phosphate buffer (PBS) containing glucose at 37° for 3-4h, yielded the high-ATP cells with 2-3 fold increase of ATP contents, while that of the erythrocytes with inosine and pyruvate under the same condition as above yielded the high-2, 3-diphosphoglycerate (DPG) cells with 4-7 fold increase of DPG contents. Similar Incubation with all these constituents together gave the high-ATP-DPG cells with similar increase of both ATP and DPG contents. The shape of these treated cells observed under scanning electron microscope was quite normal.<BR>The raised ATP level of high-ATP and high-DPG cells was maintained almost constant upon incubation at 37° in glucose-PBS for 4 h and decreased moderately upon preservation at 4° for 4 weeks. In contrast, the raised DPG level was decreased upon incubation or preservation at the normal or higher rate, although higher-than-normal amount of DPG was still kept in the cells stored at 4° for 4 weeks, due to the very high initial DPG level. The rates of decrease of ATP and DPG in the high-ATP-DPG cells were almost the same as the ATP decrease of the high-ATP cells and the DPG decrease of the high-DPG cells, respectively, indicating that the increased ATP and DPG in the high-ATP-DPG cells were metabolized independently without interfering each other. The amount of lactate produced and the medium pH drop during the preservation of these treated cells were similar to those of the untreated cells. The diminished DPG level in the ATP- and DPG-depleted erythrocytes, prepared from fresh cells by incubating them in PBS at 37° for 36 h, was improved by the treatment of the cells with adenine, inosine and pyruvate in glucose-PBS. Such a rejuvenizing treatment, however, failed to improve the decreased ATP level and osmotic resistance, and the sphero-echinocytic shape in the case of the cells extremely depleted of ATP.<BR>These evidences suggest that initial treatment of the fresh cells to raise the ATP and DPG level may be more effective in keeping the freshness of the cells in the course of the long period of the storage than the rejuvenizing treatment of the stored cells already extremely depleted of these components.
Japan Society of Clinical Chemistryの論文

Characteristics of Human Erythrocytes with Artific Ally-Increased ATP and/or 2, 3-Diphosphoglycerate Contents

スポンサーリンク

概要

著者

関連論文

スポンサーリンク