Single Camera System for Multi-Parametric Fluorescence Imaging of the Heart

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Optical mapping has become a typical method in cardiac electrophysiology. A single parameter is typically mapped, and recently dual mapping systems have been reported that record fluorescence signal of membrane potential and intracellular calcium using two cameras and a dichroic mirror. There are additional pairs of cardiac parameters measured by dual mapping system. However, the method to measure more than three parameters does not exist. To exploit the full potential of fluorescence imaging, an innovative method to measure multiple, more than three parameters is needed. In this study, we present new optical mapping system which records multiple, potentially more than three parameters using a single camera. The system consists of one camera, custom optical lens system, and filter wheel. The optical lens system is designed to focus the fluorescence light at filter position, and form an image on camera’s CMOS sensor. Filter wheel is rotated by a motor, which allows filter switching corresponding with needed wavelength. The camera exposure and filter switching are synchronized by phase locked loop techniques. We validated system performance by simple experiments; lens resolution, quantity of light, and synchronization. Using this system, we observed membrane potential and intracellular calcium in Langendorff perfused rabbit heart.