ヒト子宮内膜癌培養細胞Ishikawaおよびヒト乳癌培養細胞MCF-7に対するProgestogens効果の基礎的検討

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The effects of Medroxyprogesterone acetate (MPA) and progesterone (P) on DNA and RNA contents of a human endometrial cancer cell line (Ishikawa) and a human breast cancer cell line (MCF-7) were studied using Flow cytometry. The results were as follows. 1) MPA and P at a high dose of 10-5M inhibited their cell cycle progression through the G1 phase or G1-S phase boundary and showed a decrease in the number of cells which underwent DNA synthesis and mitosis. 2) P at a low dose of 10-7M showed the lengthening of cell cycle time in the S phase. MPA at 10-7M had an inhibitory effect on the cell cycle progression of Ishikawa cells similar to that of a high dose of MPA whereas, in the case of MCF-7 cells, it showed a lengthening of duration in the S phase. Therefore, the dose-responsibility of these cells to MPA were shown to be different. 3) A high dose of MPA and P showed a decrease in the cytoplasmic and nuclear RNA contents. A low dose of MPA and P had no marked effect on the RNA contents. From these results, it was clarified that MPA, especially at a high dose, could substantially inhibit RNA synthesis and DNA synthesis with a delay of the cell cycle progression in hormone responsive cancer cells.
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