Quantification of Periodontopathic Bacteria in Saliva Using the Invader Assay
スポンサーリンク
概要
- 論文の詳細を見る
When quantifying periodontopathic bacteria, it is important to use a convenient method that does not produce false negative results. The Invader assay is a convenient method because it does not involve gene amplification. The purpose of this study was to evaluate the validity of the Invader assay to quantify periodontopathic bacteria. The Invader technology was applied in quantifying five periodontopathic bacteria (Aggregatibacter actinomycetemcomitans, Porphyromonas gingivalis, Tannerella forsythia, Prevotella intermedia, and Treponema denticola). The Invader assay produced a linear quantitative detection range over concentrations spanning seven exponential values, with a detection limit of 103.7 copies/tube and intra-day and inter-day variance of 0.1% to 4.7% and 0.1% to 3.4%, respectively, in quantifying five periodontopathic bacteria. We compared the results of the Invader assay with those of real-time polymerase chain reaction (PCR) performed for quantifying five periodontopathic bacteria in 22 patients with periodontitis. Among the Invader-detectable bacterial strains of each species, significant correlations were observed in the counts of concerned bacterial species between these two methods, with correlation coefficients ranging from 0.757 to 0.996. This study validated repeatability and reproducibility of the Invader assay in quantifying periodontopathic bacteria and demonstrated consistent agreement between the Invader assay and real-time PCR in quantifying periodontopathic bacteria.
著者
-
Hanada Nobuhiro
Department Of Oral Health National Institute Of Public Health
-
Nomura Yoshiaki
Department Of Oral Health National Institute Of Public Health
-
Tada Akio
Department Of Applied And Environmental Chemistry Kitami Institute Of Technology
-
Takeuchi Hiroaki
Department Of Applied Chemistry School Of Science And Engineering Waseda University
-
Tanaka Kazuya
Clinical Genomics Development, BML, Inc.
-
Shimizu Hajime
Clinical Genomics Development, BML, Inc.
-
Tadokoro Kenichi
Clinical Genomics Development, BML, Inc.
-
Kawamura Katsumi
Clinical Genomics Development, BML, Inc.
-
Yamaguchi Toshikazu
Clinical Genomics Development, BML, Inc.
-
Egashira Toru
Clinical Genomics Development, BML, Inc.
-
Hanada Nobuhiro
Department of Translational Research, Tsurumi University, School of Dental Medicine
-
Tada Akio
Department of Health Science, Hyogo University
-
Takeuchi Hiroaki
Department of Translational Research, Tsurumi University, School of Dental Medicine
関連論文
- Cloning and Expression of the Mutanase Gene of Paenibacillus humicus from Fermented Food
- Surface Properties of Resin Composite Materials Relative to Biofilm Formation
- Evaluation of risk factors for dental caries from 6 to 8 years old children
- A randomized controlled trial by the 3DS for dental caries
- Fluoride-containing toothpastes available in two European countries
- Preliminary study of the short tandem repeat region of human growth hormone gone in Japanese
- Fluoride-containing mouthrinses in three European countries
- Correlation of cariogenic bacteria and dental caries in adults
- Screening of periodontitis with salivary enzyme tests
- Correlation between patient satisfaction and dental clinic credibility in regular dental check-ups in Japan
- Characteristics and willingness of patients to pay for regular dental check-ups in Japan
- Correlation study on oral health and electrocardiogram abnormalities
- 人は血管とともに老いる!--超高齢化時代に歯科医師が持つべき12の視点(新連載・1)アンチエイジングとは血管を守ること
- Electromagnetic Shielding Capacity of Wood Char Loaded with Nickel
- Successful treatment of primary central nervous system lymphoma in an elderly patient
- Oral health status and health-related quality of life: a systematic review
- Survey of dental hygienists and healthcare workers for microorganisms in the oral cavity
- Laboratory and Epidemiology Communications: Mutants Streptococci and Lactobacillus as Risk Factors for Dental Caries in 12-Year-Old children
- A Test Structure for Two-Dimensional Analysis of MOSFETs by Hot-Carrier-Induced Photoemission(Microelectronic Test Structures)
- Use of the highly sensitive PCR method to detect the Herpes Simplex Virus Type 1 genome and its expression in samples from Behcet disease patients
- 人は血管とともに老いる!--超高齢化時代に歯科医師が持つべき12の視点(2)血管内皮機能が歯周病治療で回復する(持続性炎症による血管内皮細胞の老化を含めて)
- A Case of Heparin-Induced Thrombocytopenia With Sepsis and Congestive Heart Failure : First Autopsy Report in Japan
- EPSTEIN-BARR VIRUS GENOME IN INFECTED CELLS AND CANCER
- Helicobacter pylori CagA status associated with gastric cancer incidence rate variability in Costa Rican regions
- 唾液検査標準化に関する研究 (8020推進財団 指定研究事業報告)
- 8020推進財団設立10周年記念 鼎談 10th Anniversary 8020健康長寿社会は実現するか--歯科医学・疫学研究の成果と展望
- Cell Elongation and Cell Death of Helicobacter pylori Is Modulated by the Disruption of cdrA (Cell Division-Related Gene A)
- Cloning, polymorphism, and inhibition of β-carbonic anhydrase of Helicobacter pylori
- Water Durability of Resin Bond to Pure Gold Treated with Various Adhesion Promoting Thiirane Monomers
- Effectiveness of a radiology-anatomy instructional module in a clinical course on oral radiology
- Wireless capsule endoscopy in pediatric patients : the first series from Japan
- Selective α-Glucosylation of Eugenol by α-Glucosyl Transfer Enzyme of Xanthomonas campestris WU-9701(ENZYMOLOGY, PROTEIN ENGINEERING, AND ENZYME TECHNOLOGY)
- Paraganglioma of the Cauda Equina:MR findings
- Selective Suppression of Undesirable Competitive Oxidation during Selective Catalytic Reduction of Nitrogen Monoxide by Ethene in Excess Oxygen over Metal-Loaded Aluminas
- 人は血管とともに老いる!--超高齢化時代に歯科医師が持つべき12の視点(3)口腔と全身の健康との関係(その1)
- 口のなかから体を健康にする--細菌叢の力 (特集 ヒト--細菌共生系の驚き)
- Prognostic significance of microvessel density determined by an anti-CD105/endoglin monoclonal antibody in astrocytic tumors : Comparison with an anti-CD31 monoclonal antibody
- Epstein-Barr Virus-Associated Primary Central Nervous System Lymphoma in the Japanese Population
- Thymic mucosa-associated lymphoid tissue lymphoma associated with bilateral orbital pseudotumor
- Astroblastoma: Immunohistochemical and ultrastructural study of distinctive epithelial and probable tanycytic differentiation
- Establishnent of model animal for oral infection using nonobese diabetic (NOD) mice.
- 人は血管とともに老いる!--超高齢化時代に歯科医師が持つべき12の視点(4)口腔と全身の健康との関係(その2)
- CD98 immunoreactivity in multinucleated giant cells of glioblastomas: An immunohistochemical double labeling study
- A report of supratentorial leptomeningeal hemangioblastoma and a literature review
- Production of Fab Fragment Corresponding to Surface Protein Antigen of Streptococcus mutans Serotype c-Derived Peptide by Escherichia coli and Cultured Tobacco Cells(PLANT BIOTECHNOLOGY)
- 地域住民を対象とした歯間ブラシの使用に重点をおいた歯周病予防のための健康教育プログラムの効果
- Dural Sinus Thrombosis With Marked Enlargement of the Venous Sinus : Case Report
- FUNCTIONAL RELATIONSHIP BETWEEN FROG VITELLOGENIN AND THE APPEARANCE OF TESTIS-OVA
- Controlling cariogenic bacteria by the regular check-up system
- Heredity may be one of the etiologies of supernumerary teeth
- Validity and reliability of patient satisfaction questionnaires in a dental school in Japan
- Percentile curves for food acceptance response scores in assessing chewing functions in adults
- 人は血管とともに老いる--超高齢化時代に歯科医師が持つべき12の視点(5)これからの患者の変化とその対応
- Change in Activity of Alumina by Calcination Temperature for Selective Reduction of Nitrogen Monoxide with Ethene in Excess Oxygen
- Synergy of CoAl_2O_4 and Al_2O_3 in CoAl_2O_4-Al_2O_3 Catalysts for Selective Reduction of Nitrogen Monoxide with Ethene in Excess Oxygen
- 人は血管とともに老いる--超高齢化時代に歯科医師が持つべき12の視点(6)口腔と糖尿病
- Different frequencies of Streptococcus anginosus infection in oral cancer and esophageal cancer
- Catalytic Activities of CoAl_2O_4 for Key Reactions Related to Selective Reduction of Nitrogen Monoxide with Ethene in Excess Oxygen
- Selective Removal of Cobalt Oxide from Cobalt-loaded Alumina Catalysts and Its Effect on the Activity for Selective Catalytic Reduction of Nitrogen Monoxide by Ethene in Excess Oxygen
- Enhancing and Inhibiting Effects of SO_2 on Selective Catalytic Reduction of NO_x by Ethene over Copper-Loaded Alumina Catalysts
- Impact of routine oral care on opportunistic pathogens in the institutionalized elderly
- A survey on the risk factors for the prevalence of dental caries among preschool children in Japan
- Carbonaceous Deposit as a Preferential Reductant in the Reduction of NO with C_2H_4 in Excess Oxygen over Alumina
- High Activity of Pt/AlPO_4 Catalyst for Selective Catalytic Reduction of Nitrogen Monoxide by Propene in Excess Oxygen
- 人は血管とともに老いる--超高齢化時代に歯科医師が持つべき12の視点(7)口腔と認知症--炭水化物摂取指導は神経細胞の老化を防ぐ
- 人は血管とともに老いる--超高齢化時代に歯科医師が持つべき12の視点(8)口腔と肥満
- 人は血管とともに老いる!--超高齢化時代に歯科医師が持つべき12の視点(9)口腔と呼吸器
- 人は血管とともに老いる!--超高齢化時代に歯科医師が持つべき12の視点(11)人間は何歳まで生きられるか--歯科医学によるアンチエイジングの理解
- Longitudinal Evaluation of Multi-phasic, Odontological and Nutritional Associations in Dentists (LEMONADE Study) : Study Design and Profile of Nationwide Cohort Participants at Baseline
- Effect of bovine lactoferrin on enamel demineralization and acid fermentation by Streptococcus mutans
- In vivo Dentin Caries Model using Rat Molars
- 人は血管とともに老いる!--超高齢化時代に歯科医師が持つべき12の視点(10)口腔とがん
- Prognostic significance of the immunohistochemical expression of O^6-methylguanine-DNA methyltransferase, P-glycoprotein, and multidrug resistance protein-1 in glioblastomas
- Usefulness of Intraoperative Computed Tomography in Surgery for Low-Grade Gliomas : a Comparative Study Between Two Series Without and With Intraoperative Computed Tomography
- Construction of a dental caries prediction model by data mining
- Long-term regional bond strength of three MMA-based adhesive resins in simulated vertical root fracture
- 食品のゆで時間, または刻み目がヒトの咀嚼行動に及ぼす影響
- The mechanical binding strengths of Helicobacter pylori BabA and SabA adhesins using an adhesion binding assay-ELISA, and its clinical relevance in Japan
- この人に聞く 研・究・最・前・線 歯と健康をめぐる栄養学の課題 歯科医院を「街の健康ステーション」に : 鶴見大学歯学部探索歯学講座 教授 花田信弘氏
- Feasibility of eradication of mutans streptococci from oral cavities
- 人は血管とともに老いる! : 超高齢化時代に歯科医師が持つべき12の視点(12・完)口腔細菌と腸内細菌を管理してアンチエイジングに貢献しよう
- 科学読み物 バクテリアセラピーとお口の健康
- 唾液マーカーと口腔内自覚症状との関係 : 歯周病スクリーニングにおける予備的研究
- Quantification of Periodontopathic Bacteria in Saliva Using the Invader Assay
- 全身の血管を守るために歯科医院でできること
- In Vitro Anti-Helicobacter pylori Activity of Chinese Chive (Allium tuberosum)
- B-63 チンパンジーの口腔内状態の調査 : う蝕・歯の摩耗・歯周炎・噛み合わせの評価を中心に
- Biochemical and pathophysiological characterization of Helicobacter pylori asparaginase
- B-54 チンパンジーの口腔内状態の調査 : う蝕・歯の摩耗・歯周炎・噛み合わせの評価を中心に
- Structural relationship of child behavior and its evaluation during dental treatment
- Basic Properties of Metal Phosphates and Their Catalytic Activity in the Decomposition of Diacetone Alcohol
- Functional Properties of Maillard Reaction Products in Food: Antimicrobial Activity of Aminoreductone against Pathogenic Bacteria
- Intestinal morphology in oily bitterlings (Cyprinidae : Acheilognathinae)
- Activity of Aminoreductone against Pathogenic Bacteria