Phosphoenolpyruvic Acid, an Intermediary Metabolite of Glycolysis, as a Potential Cytoprotectant and Anti-oxidant in HeLa Cells
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概要
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This study examined the cytoprotective and anti-oxidative properties of phosphoenolpyruvic acid (PEP), a glycolysis metabolite with a high-energy phosphate group. PEP (0.1–10 mM) significantly attenuated the decrease in cell viability induced by hydrogen peroxide (H2O2) in HeLa cells in a dose-dependent manner. PEP also inhibited the decrease in calcein-acetomethoxy-stained cells and the increase in propidium iodide-stained cells that were induced by H2O2. The H2O2-stimulated increase in intracellular reactive oxygen species was significantly reduced by PEP. PEP also demonstrated scavenging potential against hydroxyl radicals, as assessed by the electron paramagnetic resonance method. In addition, PEP demonstrated scavenging potential against the 1,1-diphenyl-2-picrylhydrazyl radical, a representative artificial radical, although the potential is very weak. PEP (10 mM) slightly inhibited the decrease in cellular ATP content induced by H2O2, but did not show any effects at low doses (0.1, 1 mM). PEP (0.1–10 mM) also attenuated the cell injury but not the decrease in intracellular ATP content, induced by 2-deoxy-D-glucose, a glycolysis inhibitor. These results indicate that PEP exerts cytoprotective effects and has anti-oxidative potential, although the precise cytoprotective mechanisms are not fully elucidated. We suggest that PEP is a functional carbohydrate metabolite with cytoprotective and anti-oxidative activity, and is potentially useful as a therapeutic agent against diseases that involve the oxidative stress.
著者
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Ishitsuka Yoichi
Department of Clinical Chemistry and Informatics, Graduate School of Pharmaceutical Sciences, Kumamo
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Irikura Mitsuru
Department of Clinical Chemistry and Informatics, Graduate School of Pharmaceutical Sciences, Kumamo
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Irie Tetsumi
Department Of Clinical Chemistry & Informatics Kumamoto University
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TANAKA Yuta
Department of Metallurgy, Division of Biomaterials, Institute of Biomaterials and Bioengineering, To
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KADOWAKI Daisuke
Department of Biopharmaceutics, Graduate School of Pharmaceutical Sciences, Kumamoto University
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Hamasaki Naotaka
Department Of Biochemistry Fukuoka University School Of Medicine
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Kuroda Masataka
Department Of Anesthesiology Gunma University Graduate School Of Medicine
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Kondo Yuki
Department Of Agrobioscience Graduate School Of Agricultural Science Kobe University
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Maruyama Toru
Department Of Bioclimatology And Medicine Medical Institute Of Bioregulation Kyushu University
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Nagatome Minako
Department of Clinical Chemistry and Informatics, Graduate School of Pharmaceutical Sciences, Kumamo
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Hirata Sumio
Center for Clinical Pharmaceutical Sciences, Faculty of Pharmaceutical Sciences, Kumamoto University
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Irie Tetsumi
Department of Clinical Chemistry and Informatics, Graduate School of Pharmaceutical Sciences, Kumamoto University
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Sato Keizo
First department of Biochemistry, School of Pharmaceutical Sciences, Kyushu University of Health and Welfare
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Irikura Mitsuru
Department of Clinical Chemistry and Informatics, Faculty of Medical and Pharmaceutical Sciences, Kumamoto University
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Hamasaki Naotaka
Department of Clinical Chemistry and Laboratory Medicine, Nagasaki International University
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Tanaka Yuta
Department of Clinical Chemistry and Informatics, Graduate School of Pharmaceutical Sciences, Kumamoto University
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Ishitsuka Yoichi
Department of Clinical Chemistry and Informatics, Faculty of Medical and Pharmaceutical Sciences, Kumamoto University, Japan
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