Phosphoenolpyruvic Acid, an Intermediate of Glycolysis, Attenuates Cellular Injury Induced by Hydrogen Peroxide and 2-Deoxy-D-glucose in the Porcine Proximal Kidney Tubular Cell Line, LLC-PK1

スポンサーリンク

概要

• 論文の詳細を見る

• This study was conducted to clarify whether phosphoenolpyruvate (PEP), an intermediate substance of glycolysis, has the potential to attenuate cellular injury induced by oxidative stress or dysfunctions in energy metabolism in vitro. PEP (0.5-10 mM) attenuated hydrogen peroxide (H2O2)-induced cellular injury in the porcine proximal kidney tubular cell line, LLC-PK1 in a dose-dependent manner. PEP also prevented cellular injury in LLC-PK1 cells induced by the glycolysis inhibitor, 2-deoxy-D-glucose (2-DG). In addition, PEP significantly enhanced the degradation of H2O2. The prevention of H2O2-induced cellular injury mediated by PEP was more potent than that of the carbohydrates, glucose and trehalose, which are used as components of organ preservation solutions for clinical transplantation. In conclusion, we demonstrated that PEP is a bifunctional carbohydrate with anti-oxidant properties and suggest that PEP is potentially useful as an organ preservation agent in clinical transplantation.

• 公益社団法人　日本薬学会の論文

著者

• Ishitsuka Yoichi

  Department of Clinical Chemistry and Informatics, Graduate School of Pharmaceutical Sciences, Kumamo

• Irikura Mitsuru

  Department of Clinical Chemistry and Informatics, Graduate School of Pharmaceutical Sciences, Kumamo

• Irie Tetsumi

  Department Of Clinical Chemistry & Informatics Kumamoto University

• KADOWAKI Daisuke

  Department of Biopharmaceutics, Graduate School of Pharmaceutical Sciences, Kumamoto University

• Hamasaki Naotaka

  Department Of Biochemistry Fukuoka University School Of Medicine

• Ishitsuka Yoichi

  Dep. Of Clinical Chemistry And Informatics Graduate School Of Pharmaceutical Sciences Kumamoto Univ.

• Kuroda Masataka

  Department Of Anesthesiology Gunma University Graduate School Of Medicine

• Kondo Yuki

  Department Of Agrobioscience Graduate School Of Agricultural Science Kobe University

• Nagatome Minako

  Department of Clinical Chemistry and Informatics, Graduate School of Pharmaceutical Sciences, Kumamo

• Saisho Yusuke

  Department of Clinical Chemistry and Informatics, Graduate School of Pharmaceutical Sciences, Kumamo

• Hirata Sumio

  Center for Clinical Pharmaceutical Sciences, Faculty of Pharmaceutical Sciences, Kumamoto University

• Kondo Yuki

  Department of Clinical Chemistry and Informatics, Graduate School of Pharmaceutical Sciences, Kumamoto University

• Irie Tetsumi

  Department of Clinical Chemistry and Informatics, Graduate School of Pharmaceutical Sciences, Kumamoto University

• Irikura Mitsuru

  Department of Clinical Chemistry and Informatics, Faculty of Medical and Pharmaceutical Sciences, Kumamoto University

• Irikura Mitsuru

  Department of Clinical Chemistry and Informatics, Graduate School of Pharmaceutical Sciences, Kumamoto University

• Nagatome Minako

  Department of Clinical Chemistry and Informatics, Graduate School of Pharmaceutical Sciences, Kumamoto University

• Saisho Yusuke

  Department of Clinical Chemistry and Informatics, Graduate School of Pharmaceutical Sciences, Kumamoto University

• Hamasaki Naotaka

  Department of Clinical Chemistry and Laboratory Medicine, Nagasaki International University

• ISHITSUKA Yoichi

  Department of Clinical Chemistry and Informatics, Graduate School of Medical and Pharmaceutical Sciences, Kumamoto University

• Ishitsuka Yoichi

  Department of Clinical Chemistry and Informatics, Graduate School of Pharmaceutical Sciences, Kumamoto University

• Kuroda Masataka

  Department of Clinical Chemistry and Informatics, Graduate School of Pharmaceutical Sciences, Kumamoto University

• Ishitsuka Yoichi

  Department of Clinical Chemistry and Informatics, Faculty of Medical and Pharmaceutical Sciences, Kumamoto University, Japan

関連論文

• The Ethical Kampo Formulation Sho-Seiryu-To (TJ-19) Prevents Bleomycin-Induced Pulmonary Fibrosis in Rats
• Proteolytic Cleavage Sites of and 3 Protein in Alkali-Treated Membranes: Fidelity of Hydropathy Prediction for Band 3 Protein^1
• A Structural Study of the Carboxyl Terminal Region of the Human Erytheocyte Band 3 Protein
• Biological Immunoassay Utilizing Magnetic Marker and High T_c Superconducting Quantum Interference Device Magnetometer
• Synthesis of Second Transmembrane Segment Peptides in Band 3 Protein from Human Erythrocyte Membrane and Their Interaction with Model Membranes
• The C-terminal Tail of Mitochondrial Transcription Factor A Markedly Strengthens its General Binding to DNA
• PDIP38 Associates with Proteins Constituting the Mitochondrial DNA Nucleoid
• HIGH INCIDENCE OF A POLYMORPHIC VARIANT OF ERYTHROCYTE MEMBRANE PROTEIN, BAND 3-MEMPHIS, ON A WESTERN JAPANESE ISLAND
• Effect of Olmesartan on Oxidative Stress in Hemodialysis Patients
• Treatment with Crystalline Ultra-Pure Urea Reduces the Aggregation of Integral Membrane Proteins without Inhibiting N-Terminal Sequencing
• Mutation Detection in the Drug-Resistant Hepatitis B Virus Polymerase Gene Using Nanostructured Reverse Micelles
• Characterization of IL-4 and IL-13 signals dependent on the human IL-13 receptor α chain 1: redundancy of requirement of tyrosine residue for STAT3 activation
• Position-independent human β-globin gene expression mediated by a recombinant adeno-associated virus vector carrying the chicken β-globin insulator
• A Selective Thromboxane A_2 (TXA_2) Synthase Inhibitor, Ozagrel, Attenuates Lung Injury and Decreases Monocyte Chemoattractant Protein-1 and Interleukin-8 mRNA Expression in Oleic Acid-Induced Lung Injury in Guinea Pigs
• Effects of Bolus Injection of Soybean-Based Fat Emulsion and Fatty Acids on Pulmonary Gas Exchange Function(Pharmacology)
• Preventive Effect of Tranilast on Oleic Acid-Induced Lung Injury in Guinea Pigs(Pharmacology)
• Oxidative Stress in Early Stage of Acute Lung Injury Induced with Oleic Acid in Guinea Pigs
• Preventive Effect of Phosphoenolpyruvate on Hypoxemia Induced by Oleic Acid in Guinea Pigs
• Involvement of Calmodulin Inhibition in Analgesia Induced With Low Doses of Intrathecal Trifluoperazine
• Secondary Structures of Synthetic Peptides Corresponding to the First Membrane-Contact Portion of Normal Band 3 and Its Deletion Mutant (Southeast Asian Ovalocytosis)
• Differential effects of hyperventilation on cerebral blood flow velocity after tourniquet deflation during sevoflurane, isoflurane, or propofol anesthesia
• Redox Nature of Fe-Incorporated Magnesium ortho-Vanadate as a Catalyst for the Oxidative Dehydrogenation of Propane
• Massspectrometric Analyses of Transmembrane Proteins in Human Erythrocyte Membrane
• The Functional Role of Arginine 901 at the C-Terminus of the Human Anion Transporter Band 3 Protein
• ANDP-2, A Novel Acrinol Degradation Product by Light
• Computed tomography fluoroscopy-guided lumbar sympathectomy for a patient with peripheral vascular disease and lumbar spine compression fracture
• Semi-quantitative analysis of telomerase in pancreatic ductal adenocarcinoma
• Systemic Vasculitis Associated with α1-antitrypsin Deficiency
• Construction of an Expression System for Human α_1-Acid Glycoprotein in E. coli : the Roles of Oligosaccharide Moieties in Structural and Functional Properties
• Combined Protein C and Protein S Deficiency in a Family with Repetitive Thromboembolism and Segregated Gene Mutations
• A Selective Thromboxane A2 (TXA2) Synthase Inhibitor, Ozagrel, Attenuates Lung Injury and Decreases Monocyte Chemoattractant Protein-1 and Interleukin-8 mRNA Expression in Oleic Acid–Induced Lung Injury in Guinea Pigs
• Phosphoenolpyruvic Acid, an Intermediate of Glycolysis, Attenuates Cellular Injury Induced by Hydrogen Peroxide and 2-Deoxy-D-glucose in the Porcine Proximal Kidney Tubular Cell Line, LLC-PK1
• Synthesis and Seed Germination Stimulating Activity of Some Imino Analogs of Strigolactones
• Mechanism of the Change in Shape of Human Erythrocytes Induced by Lidocaine
• Preoperative depression is a risk factor for postoperative short-term and long-term cognitive dysfunction in patients with diabetes mellitus
• Effects of Inorganic and Organic Anions on the Transport of Phosphoenol-Pyruvate Across the Erythrocyte Membrane(MEMBRANE PROTEINS AND TRANSPORT-II)
• A Novel Function of TDIF-Related Peptides : Promotion of Axillary Bud Formation
• CLE Peptides can Negatively Regulate Protoxylem Vessel Formation via Cytokinin Signaling
• Phosphoenolpyruvic Acid, an Intermediary Metabolite of Glycolysis, as a Potential Cytoprotectant and Anti-oxidant in HeLa Cells
• Association between cerebrovascular carbon dioxide reactivity and postoperative short-term and long-term cognitive dysfunction in patients with diabetes mellitus
• Treatment with Crystalline Ultra-Pure Urea Reduces the Aggregation of Integral Membrane Proteins without Inhibiting N-Terminal Sequencing.
• Proteolytic Cleavage Sites of Band 3 Protein in Alkali-Treated Membranes: Fidelity of Hydropathy Prediction for Band 3 Protein.
• Secondary Structures of Synthetic Peptides Corresponding to the First Membrane-Contact Portion of Normal Band 3 and Its Deletion Mutant (Southeast Asian Ovalocytosis).
• Massspectrometric Analyses of Transmembrane Proteins in Human Erythrocyte Membrane

もっと見る 閉じる

スポンサーリンク