Effect of irradiation on chondrocytes in culture.
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概要
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It has been recognized that irradiation causes damage to the growth of osseous and cartilagenous tissue. In this study, the effect of irradiation on chondrocytes in culture was investigated. Chondrocytes derived from 21-day-old rabbit cartilage were cultured in Ham F-12 medium supplemented with 10% FCS. The culture dishes were irradiated with a single dose of 200 rads, 1, 000 rails, and 5, 000 rads by gamma cell 40. The synthesis of DNA was inhibited for 2 days after irradiation of 200 rad, and thereafter it recovered. With irradiation of 1, 000 rails, synthesis of DNA was inhibited completely and did not recovered. Synthesis of proteoglycan was not significantly inhibited with the irradiation in this experiment.
- 西日本整形・災害外科学会の論文
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