ヘパリンを静脈注射により投与した後のテラピア血漿中のリパ-ゼ活性〔英文〕

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In mammals, it is well known that, during transport in the blood, triglyceride-rich lipoproteins (e. g. VLDL or chylomicrons) are metabolized actively by lipoprotein lipase (E. C. 3.1.1.3) in various endothelial cells and that this lipase is released into the blood stream after intravenous injection of heparin. In this work, we found that lipase activity appeared in the plasma of the fish Tilapia nilotica after intravenous injection of heparin. This lipase activity was optimal at pH 9.0-9.8 and its activity increased in the presence of NaCl (0.01-2.00M) or protamine sulfate (100-1000μg/ml). These properties are different from those of post-heparin lipoprotein lipases of mammals (e. g. rats and pigs). This fish serum contained an activator of lipoprotein lipase like apolipoprotein CII or H in human serum. The fish serum greatly increased lipoprotein lipase activity in rat epididymal adipose tissue, but inhibited the lipase activity of fish visceral adipose tissue.
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