Fluctuation of Serum Monoamine Oxidase (MAO) Activity in Fracture

概要

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A new method for estimation of serum MAO was established. In this method, benzylamine was used as substrate and ammonia liberated by action of serum MAO was measured by the direct colorimetric determination. With the method, serum MAO activity was examined in normal persons and various patients. In normal persons, serum MAO activity was high in younger generation (0 to 10 years of age), gradually decreased with aging and reached at minimum in 41 to 50 years of age. Serum MAO activity was found to be elevated at 51 to 70 years of age and then reduced over 71 years of age. In the patients, it was found that serum MAO was considerably elevated in the patients with fracture in addition to the patients with liver diseases. Then, experiments were carried out to elucidate whether or not serum MAO was the same enzyme as bone MAO. It was found that serum MAO had similar characteristics to the bone enzyme in the points of optimum pH, Km value, substrate specificity and inhibition by fβ-proprionitrile. It was also demonstrated that serum MAO was increased after fracture of rat legs. Based on these results, the physiological significance of serum MAO in fraction was discussed.
Japan Society of Clinical Chemistryの論文