Studies of the extractive components of ascidians in Korea-III. Regional variation of extractive nitrogenous constituents in the ascidian Halocynthia roretzi muscle.

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The muscle extracts of the ascidian Halocynthia roretzi cultured at the southern coast near Chungmu and the eastern coast near Pohang of Korea, were analyzed for extractive nitrogen, free and combined amino acids, nucleotides and related compounds, quaternary ammonium bases, and guanidino compounds using specimens collected in December, 1988 and in April, 1989, and com-pared for those contents with each other. The amount of extractive nitrogen fluctuated regionally in both seasons. Free amino acids, such as taurine, glutamic acid, proline, glycine, and alanine, were rich in all the samples. However, the amounts of those amino acids were considerably different between the two localities in winter and spring samples. As for nucleotides and related compounds, the regional variation was clearly observed again in both of the winter and spring samples similarly to extractive nitrogen and free amino acids. Glycinebetaine, homarine, and trigonelline were detected in all the samples, The amounts of those betaines did not differ greatly between the two spring samples, but those of the two winter samples exhibited a great discrepancy with each other.