Higher Concentrations of Interferon-γ Enhances Uptake and Transport of Dietary Antigens by Human Intestinal Cells : A Study Using Cultured Caco-2 Cells
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概要
- 論文の詳細を見る
Besides functioning as a mucosal barrier and transporting nutrients, intestinal epithelial cells (IECs) also serve as antigen-presenting cells (APCs). Modification of protein antigens by proteolysis is one of the principal steps in antigen presentation to Th cells. We used a Caco-2 intestinal epithelial cell line to investigate the transepithelial transport of the dietary antigen, ovalbumin (OVA). We also examined the effects of the proinflammatory cytokine interferon-γ (IFN-γ) on the antigen transport process in Caco-2 cell layers. Caco-2 cell layers transferred both intact and degraded OVA from the mucosal to the serosal side. IFN-γ stimulated OVA transport and most of the transported OVA in such cells were degraded. We also examined OVA uptake by Caco-2 cells using immunohistochemical means. Caco-2 cells incorporated OVA in a time-dependent manner and IFN-γ significantly enhanced antigen internalization. Flow cytometry also demonstrated that IFN-γ elevated the internalization of FITC-OVA. We also determined the effects of low and high concentrations of IFN-y on mucosal permeability and internalization of FITC-OVA. Althoughh both 10 and 50ng/mL IFN-γ stimulated mucosal permeability to the same extent, more FITC-OVA was internalized by Caco-2 cells incubated with 50 than with 10ng/mL IFN-y. These results suggest that the effects of IFN-γ on mucosal permeability and the internalization of antigens by intestinal epithelial cells are brought about by different mechanisms. Therefore, higher concentrations of IFN-γ stimulate the uptake, processing, and transport of dietary antigens by IECs.
- 財団法人 学会誌刊行センターの論文
- 2003-06-01
著者
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Takeda E
Department Of Clinical Nutrition University Of Tokushima Graduate School
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Takeda Eiji
Department Of Clinical Nutrition School Of Medicine Tokushima University
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Koda Tomoko
Department Of Clinical Nutrition School Of Medicine Tokushima University
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Koda Tomoko
Department Of Nutrition Faculty Of Human Life Sciences Yamaguchi Prefectural University
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Yamano Mariko
Department Of Rehabilitation Osaka Prefecture College Of Nursing
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Yamano Mariko
Department Of Neuroanatomy Institute Of Higher Nervous Activity Osaka University Medical School
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Ogawa Tadashi
Division Of Food Science And Biotechnology Graduate School Of Agriculture Kyoto University
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Koda T
Kyoto Univ.
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Minami Hisanori
Division Of Food And Health Environment Faculty Of Environmental And Symbiotic Sciences Prefectural
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Takeda Eiji
Department Of Applied Physics Faculty Of Engineering Osaka University
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Ogawa T
Division Of Food Science And Biotechnology Graduate School Of Agriculture Kyoto University
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Minami Hisanori
Division Of Food And Health Environment Faculty Of Environmental And Symbiotic Sciences Prefectural University Of Kumamoto
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Yamano M
Department of Rehabilitation, Osaka Prefecture College of Nursing
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