Separative determination of ascorbic acid and erythorbic acid by high-performance liquid chromatography.

概要

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High-performance liquid chromatography (HPLC) was applied to the determination of ascorbic acid (AsA) and erythorbic acid (ErA). The apparatus was a Shimadzu model LC-2P Liquid Chromatograph equipped with a UV detector set at 254nm. The separation was achieved on a LiChrosorb-NH2 column which was pre-treated with 0.1M ammonium monophosphate solution using a mixture of acetonitrile, acetic acid and water (87:2:11, v/v) as an eluant. The HPLC method has the following advantages: AsA and ErA are quantitated after being distinctly separated, analysis time per one sample is short, and AsA or ErA levels as low as 1.0×10-2μg are detectable. Recovery experiments with dehydro-AsA and dehydro-ErA, involving reduction with H2S, gave satisfactory results.
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