An improved high-performance liquid chromatographic assay for the determination of free and esterified carnitine in animal tissues.
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概要
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A high-performance liquid chromatographic procedure for the determination of carnitine that is converted to coenzyme A(CoA) stoichiometrically by carnitine acetyltransferase is described. The chromatographic separation was accomplished by using Unisil QC8 column and a mixture of 190 m t KH2PO4 and methanol (87: 13, v/v) as eluent. The present method is rapid, sensitive, and can be used for the routine analysis of animal tissues and plasma.
著者
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荒川 信彦
Department of Food and Nutrition, Ochanomizu University
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大塚 恵
Department of Food and Nutrition, Ochanomizu University
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河 台烈
Department of Food and Nutrition, Ochanomizu University
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荒川 信彦
Department of Food and Nutrition
関連論文
- Effect of Graded Doses of Erythorbic Acid on Activities of Drug Metabolic Enzyme and Phosphatases in Guinea Pigs
- Separative determination of ascorbic acid and erythorbic acid by high-performance liquid chromatography.
- Separative determination of ascorbic acid and erythorbic acid in animal tissues by high-performance liquid chromatography.
- An improved high-performance liquid chromatographic assay for the determination of free and esterified carnitine in animal tissues.
- Effect of erythorbic acid administration on activities of drug metabolic enzyme and phosphatases in guinea pigs administered an adequate amount of ascorbic acid.
- Effect of graded doses of erythorbic acid on ascorbic acid content of tissues of guinea pigs.
- Effect of erythorbic acid administration on ascorbic acid content in guinea pig tissues.
- The tissue distribution of L-ascorbic acid and dehydro-L-ascorbic acid in the guinea pigs injected intravenously with dehydro-L-ascorbic acid.
- The effect of graded doses of ascorbic acid on the tissue carnitine and plasma lipid concentrations.