グルタミン酸生産菌の変異株を用いるL-チトルリン醗酵

概要

論文の詳細を見る
An arginine-requiring mutant of a glutamic acic-producing bacterium, Corynebacterium glutamicum (syn. Micrococcus glutamicus) KY 9396, was found to accumulate a large quantity of an amino acid in the culture medium. The amino acid was isolated in crystalline form from the culture medium and was identified as L-citrulline by its melting point, elementary analysis, infrared absorption spectrum and optical rotation. The cultural conditions for L-citrulline production by this mutant were investigated. In synthetic media, arginine concentration remarkably affected L-citrulline accumlation. The L-citrulline accumulation was maximum at a suboptimum concentration of arginine for the growth of the microorganism, namely 125 micrograms per milliliter. L-Citrulline yield increased as the concentrations of potassium phosphate in the medium decreased until both mono- and dipotassium phosphate became to the level of 0.025%. It was noted that L-glutamic acid accumulation decreased with the increase of L-citrulline accumulation. Replacement of arginine in the medium with yeast extract markedly increased the L-citrulline accumulation. Thus, the amount of L-citrulline accumulation reached to a level of 10.7 milligrams per milliliter with the medium containing 10% of glucose.
社団法人日本農芸化学会の論文