Corynebacterium glutamicumのリジン要求株M-14によるL-イソロイシン生成と若干の前駆物質およびアミノ酸の効果

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An L-lysine auxotrophic mutant of Corynebacterium glutamicum M-14 was found to produce L-isoleucine at a low concentration of L-lysine. The optimum concentration of L-lysine for L-isoleucine accumulation was 10_??_20μg/ml and 2.0mg/ml of L-isoleucine was produced in a synthetic medium containing glucose (10%) as a carbon source. L-Homoserine and L-aspartic acid increased L-isoleucine production in the medium containing L-lysine at the concentration (100μg/ml as monohydrochloride) inhibitory to de novo production of L-isoleucine. Addition of L-methionine or L-threonine decreased L-isoleucine production remarkably in the medium containing L-lysine at the concentration optimal for de novo production of L-isoleucine (10μg/ml as monohydrochloride). α-Aminobutyric acid and α-hydroxybutyric acid increased the L-isoleucine production significantly and 6.2mg/ml of L-isoleucine was produced when 10mg/ml of α-hydroxybutyric acid was supplemented to the medium. The effect of α-aminobutyric acid and a-hydroxybutyric acid on the increase of L-isoleucine production was noted also in some hydrocarbon utilizing microorganisms.
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