酵化細菌に関する研究-10-

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The influences of added substances to the production of α-ketoghitaric acid from glucose were investigated by using Serratia marcescens No 18. in shaking culture. It was found that Co++ and Cd++ acted as stimulant to the α-ketoglutaric acid accumulation, the role of Co would be similar to Fe++ or Fe+++, which activate the enzyme system concerning to the α-ketoglutaric acid for-mation, and the optimum concentration lied at about 0.1_??_1 ppm. Na+ and K+ stimulated the accumulation also, but it occurred at rather high concentration of 0.1M, the action of which was thought to be different from the above metallic ions. Na arsenite (1×10-3M) resulted also the accumulation and the effect was multiplicable with the simultaneous addition of NaCl (0.1M). Thiamin, pyridoxine, nicotinic acid, nicotinic amide stimulated the accumulation, while pantothenic acid repressed, nevertheless stimulated the pyruvic acid accumulation. The addition of citrate, malate and fumarate did'nt give any remarkable effect on the α-ketoglutaric acid accumulation.
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