細菌によるDL-5-(β-メチルメルカプトエチル)-ヒダントインからL-メチオニンの生産 L-メチオニン生産菌の分離同定,生成物の分離確認および分離菌によるL-メチオニン生産至適条件の検討

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The microbial transformation of DL-5-(β-methylmercaptoethyl)-hydantoin (DL-Hyd) to L-methionine was studied. The bacteria which can convert DL-Hyd to methionine and carbamylmethionine was isolated from soil and identified as Bacillus coagulans. Among eleven strains of B. coagulans and other bacteria from IAM culture collection, none of the bacteria was capable of forming L-methionine from DL-Hyd. Methionine and carbamyl-methionine formed from DL-Hyd were identified as L- and D-form, respectively. The optimal conditions for producing L-methionine were studied, and the yield of Lmethionine and carbamyl-D-methionine attained to 56.7 and 17.6 molar percent respectively, when the bacteria was incubated aerobically at 30°C for 26 hr using the following medium: 1% of starch, 0.25% of urea, 0.8% of corn steep liquor, 0.5% of KH2PO4, 0.01% of MgSO4 and 0.001% of each Fe and Mn sulfates. The mechanism of transformation of DL-Hyd to L-methionine and carbamyl-D-methio-nine was discussed.
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