血漿の XAD-2 Resin 抽出による Cholecystokinin の Radioimmunoassay ヒトにおける食事負荷, 十二指腸内硫酸マグネシウム注入時の血中 Cholecystokinin の上昇

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A sensitive and specific radioimmunoassay of cholecystokinin (CCK) has been achieved by method of Chang and Chey. Natural porcine CCK33 was iodinated by a modification of chloramine-T method. Purification and separation of labelled CCK was carried out by two-step procedure incorporating gel filtration and ion-exchange chromatography. The labelled preparation has a specific radioactivity of 709mCi/μM. Nonspecific plasma interference was eliminated by a XAD-2 resin column extraction technique. High recovery of CCK has resulted from this procedure for extraction.The contribution of gastrin was relatively small in extracted samples. The detection limit was 3.2pM. Intra- and interassay precision was 27.0±2.4 (M±SD) pM(n=10) and 32.4±4.0 pM (n=10), respectively. Using this assay, plasma CCK concentrations in response to ingestion of a test meal were studied in 6 healthy subjects. After ingestion of a meal, the mean plasma CCK concentration increased significantly above the fasting concentration of 11.6±0.5 (M±SE) pM and remained elevated over a 2-hr period (p<0.01). In 6 healthy subjects, Meltzer-Lyon test was studied. As the flow of bile from duodenal tube increased markedly following intraduodenal ingestion of 25% MgSO4 40ml, significant increase in plasma CCK concentration was detected (p<0.05).
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