水産動物のキチン分解酵素に関する研究-2-

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In the previous paper, 1) by the present authors it was reported that the chitinolytic enzyme was present in the stomach of Japanese sea-bass, Lateolabrax japonicus and suggested that the enzyme was inherent in the fish itself. The present study was carried out in order to ascertain whether the chitinolytic enzyme was present in the liver or not. In order to determine chitinolytic system, water extracts of the liver were incubated with a chitin suspension, then chitobiose and N-acetylamino sugar liberated were determined. The amount of N-acetylamino sugar was measured by the method as described by REISSIG et al.4) Chitobiose was estimated from the amount of N-acetylamino sugar produced by the addition of β-glucosidase.5) Results obtained are as follows. 1) The chitinolytic enzyme is present in the liver of Japanese sea-bass. 2) Substances which are formed by the action of the chitinolytic enzyme may be N-acetylglucosamine and N, N'-diacetylchitobiose (Fig. 1), and the enzyme may consist of chitinase and chitobiase. 3) The optimum pH value of chitinase action is about 4 and the optimum temperature is at 40 ?? 50°C. The optimum pH value of chitobiase is also about 4 (Fig. 3, Fig. 4 and Fig. 6).
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