Affect of cytochalasin B (CB) and phalloidin (Ph) to the actin filaments of primary cultured rat hepatocyte.

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The alterations of actin filaments were observed using doublet cells in primary cultured rat hepatocytes and were examined the effects of 50μM cytochalasin B and 1.27μM phalloidin. The observation was performed by phase contrast micrographs and fluorescent micrographs by means of DACM-HMM. In normal control, after 3 hours incubation, actin filaments were observed especially around the bile canaliculi between two hepatocytes. After 24 hours incubation, hepatocytes showed spreading and actin filaments bundles were appeared in the cytoplasm.<BR>In CB treated group, hepatocytes did not show spreading even after 24 hours, and granular actin filaments were identified around the widely dilated bile canaliculi, but no actin filaments bundles were observed in the cytoplasm during the observation.<BR>In Ph treated group, we observed the formation of protrusions from free cell surface membrane and hepatocytes did not show spreading even 24 hours incubation. In DACM-HMM fluorescent micrograph, actin filaments could be seen around the bile canaliculi between two hepatocytes. After 24 hours, the specific fluorescence around the bile canaliculi were increased, and granular actin filaments appeared in the cytoplasm. But normal actin filaments bundles did not appeare in the cytoplasm during the observation.
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