Three dimentional examination on the hepatocyte cytoskeleton of obstructive jaudice liver by quick freezing and deep-etching replica method.

概要

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In order to examine three dimentional changes on hepatocyte cytoskeleton of obstructive jaundice liver, we observed the hepatocytes in common bile duct ligated rats by quick freezing and deep-etching replica method as well as conventional transmission electron microscopy. Networks of bile canalicular microfilaments (BCMF) proliferated around dilatated bile canaliculi and spread to the surroundings of neiboring junctional complexes. BCMF attached directly to bile canalicular membrane in one end and similar (end-to-membrane) to the proliferated intermediate filaments (IF) and vesicles in another. Predominantly increased IF ran parallel and attached directly to junctional complexes, and seemed to reinforce them. IF also increased extremely at the peripheral cytoplasm of hepatocytes sometimes in bundle forms. It is suggested that these findings are the adaptation of hepatocyte cytoskeleton to remarkably increased bile duct pressure and cholestasis. To the contrary, arrangement of organelle and cytoskeleton at the cytoplasm near nucleus were preserved almost intact on three dimentional architecture.