肝疾患におけるエンドトキシン血症-測定法の吟味と新しい血漿処理法による成績-

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We measured plasma endotoxin in 47 patients with chronic liver disease by The chromogenic substrate assay after conventional and our improved plasma treatment. Endotoxin in the precipitate after the perchloric acid treatment was neutralized and solubilized by adding triethylamine. Endotoxin concentrations in the supernatant and precipitate were measured by substrate S-2423, Toxicolor test<SUP>®</SUP> and Endospecy<SUP>®</SUP>. Irrespective of the kind of substrate the recovery rate of endotoxin in the supernatant in the conventional acid method was much lower than that in the heating method. In cirrhotics a large amount of endotoxin was detected in the precipitate. When total endotoxin concentraiton both in the supernatant and the precipitate was calculated by our new acid method, it was correlated negatively to serum albumin, cholinesterase, HDL-cholesterol and transferrin and was correlated positively to serum total bilirubin in chronic alcoholics. Endotoxemia detected by our new acid method may reflect spillover endotoxemia in chronic alcoholics.
社団法人日本肝臓学会の論文