ABSORPTION LOSS OF PEPTIDES TO THE PLASTIC TUBE IN RADIORECEPTOR ASSAY OF CALCITONIN AND β-ENDORPHIN: PROTECTION BY DETERGENTS
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概要
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During development of the calcitonin binding assay using rat brain particulate fraction, it was noticed that <SUP>125</SUP>I-salmon calcitonin-I (<SUP>125</SUP>I-SCT) was partly absorbed by the polypropylene tube surface in an irreversible manner. Absorption was partially prevented by cold SCT and complete prevention was achieved by bacitracin, Triton X-100, Brij 36T, and some Zwittergents in such concentration ranges which appeared not to disrupt the <SUP>125</SUP>I-SCT binding ability of brain tissue. The brain fraction itself exhibited a similar preventive effect. The anti-absorbing effect of Brij 36T was also observed in an opiate receptor binding assay for β-endorphin. These results led us to recommend that the binding assay for these peptides should be done only in the presence of an appropriate antiabsorbant.
- 社団法人 日本薬理学会の論文
著者
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NAKAMUTA HIROMICHI
Department of Parmacology, Setsunan University
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Koida M
Department Of Pharmacology Faculty Of Pharmaceutical Sciences Setsunan University
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Koida Masao
Department Of Parmacology Setsunan University
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MATSUO Junko
Department of Pharmacology, Faculty of Pharmaceutical Sciences, Nagasaki University
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YAMAMOTO Yoshinobu
Department of Pharmacology, Faculty of Pharmaceutical Sciences, Nagasaki University
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Nakamuta Hiromichi
Department Of Parmacology Setsunan University
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Matsuo Junko
Department Of Mental Disorder Research National Institute Of Neuroscience National Center Of Neurology And Psychiatry
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