アレルギー患者における好塩基球脱顆粒試験

概要

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The basophil which bears specific IgE degranulates and disappears after challenge with allergen. This phenomenon was used as diagnostic test of allergies. A human basophil degranulation test (HBDT) was carried out on a slide using an enriched cell suspension. On each slide, 20μl of enriched cell suspension and 10μl of the allergen or, as a contaol, 10μl of buffer solution were placed in wells. After fixed and stained, the basophil count was made on an equal number of random distributed microscopic fields. When numbers of basophil decreased more than 35% in allergen wells, HBDT was determined as positive.In the present report, five allergens were included, namely house dust (HD), mites (mixture of Dermatophagoides pteronyssinus and D. farinae), pollen, hairs of cat and dog. In all allergens, there were good correlations between results of HBDT and skin test, and the concordance rate were 69.2% in HD, 80.8% in mites, 90.5% in pollen and 91.7% in cat and dog. There was also good correlation between the results of HBDT and RAST score. Its concordance reached 91%.This HBDT is plain and simple, and shows similar reliability to RAST. HBDT will prove to be an additionally useful diagnostic clue for allergy.
社団法人日本耳鼻咽喉科学会の論文