Paper-Chromatographic Method of Tea Catechins

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A tentative method for paper chromatographic study of tea catecbins was established after several meetings of tea chemical engineers. The procedure is as follows : 1. Preparation of sample solution. Add 10 c.c. of boiling water to 1g. of sample, keep in a boiling water bath for 5min. Drop 0.02 c.c. of this infusion on the original point of filter paper. 2. Two dimensional development. Filter paper : Toyo No.3, 20×20cm. Developing temperature : 20°C (Unless 20°C. adopted, describe the temperature used.) Original point of development : Near a corner of the paper 2cm from either edge. The-first run : Developing solvent : Phenol containing 25% (vol.) water. Developing distance : Reach the solvent front 2cm, below the upper edge of the paper. Developing direction : Along the fibre arrangement, of the paper. After the first run is over, dry the paper in an electric heater, cut off the contaminated part of the paper by phenol front. The second run : After standing one night, perform the second run. Developing solvent : n-Butanol : acetic acid : water (4:1:2 vol.). Developing distance : Same length as the first run. Coloring reagent : A 1% solution of MOHR'S salt plus same volume of methanol.