The Influence of Light upon the Chemical Constituents of Tea Seedling
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Tea seeds were sawn in sand in the dark or in the light. After germination the chemia constituents of the seedlings were compared.<BR>Caffeine, a specific component of tea, was proved to be not contained in the root and seed, nevertheless it was contained 1.8-3 times as much in the leaf and stem grown in the dark as in the light. It was confirmed that the caffeine synthesis did not necessitate sunlight.<BR>The nitrogenous constituents were contained most abundantly. in the leaf, and more less in the root, stem, and cotyledon in the decreasing order.<BR>As for the composition of nitrogen fractions, one-third of nitrogen in the leaf or stem in the light, half of nitrogen in the leaf in the dark and in the root in the light, two-thirds of nitrogen in the cotyledon in the dark or light, and three-forths of nitrogen in the stem and root in the dark were in soluble form. The dark treatment generally increased the soluble nitrogen/total nitrogen ratio in the nitrogen fractions. And the caffeine-free soluble fraction was remarkably variable in the soluble nitrogen fractions.<BR>From the calculation of the weight of each fraction per seedling, it was presumed that the amount of nitrogen in each fraction in the seedling grown in the dark was nearly equal to that of the corresponding fraction in the seed, while in the light one-tenth of the soluble nitrogen in the seed and one-third of that migrated into the seedling and transformed to the caffeine nitrogen and insoluble nitrogen respectively.<BR>Sixteen spots of amino acids and amides except unidentified ones were detected by paper-chromatography. Theanine, a specific amide of tea, was contained in a very small amount in the seed, but after germination appeared in every part of seedling grown both in the dark and in the light, and especially occurred in the root most abundantly. Alanine, aspartic acid and arginine were also found in every part in all cases, and the former two were contained abundantly in the leaf and stem, the latter in the root respectively. Glutamine and threonine were presumed to be prevailing components and the former was found in large quantity in the stem, the latter in all parts of seedling grown in the dark. Asparagine had a trend to be contained abundantly in the dark.<BR>As for catechins, the leaf in the light contained (-)-epigallocatechin and its gallate, (-)-epicatechin gallate. (±)-gallocatechin, gallic acid and theogallin. The former two occurred more abundantly. The light→dark treatment reduced catechins, especially (-)-epigallocatechin gallate was decreased.
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- Estimation of Debiraki-Day of Tea Shoot
- Growth Promoting Effect of Gibberellin upon the Tea Shoot (Continued)
- Preliminary Surveys on the Diagnosis of Tea Plant by the Foliar Analysis
- The Influence of Light upon the Chemical Constituents of Tea Seedling
- A Consideration upon the Adjustment of Yield of Tea Leaves:With Special Reference to the Maturity of Leaves
- Growth Promoting Effect of Gibberellin upon the Tea Shoot
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